Glutamate suppression of feeding and the underlying output of effector neurons in Helisoma

This study tested the consequences of 3 stress conditions on feeding consumption in the freshwater snail Helisoma. Two stresses (placement in a hypotonic environment and body wall incision) were without effect on food consumption. In contrast, placement of animals in a hypertonic environment (20% seawater) caused a transient suppression of feeding. Since the osmolarity and composition of molluscan blood is known to be altered under conditions of osmotic stress, we reasoned that a blood-borne agent might be responsible for the observed suppression of feeding. We tested this hypothesis by chromatographic analysis of blood and, based on this analysis, subsequent assay of putative neuromodulatory agents on the patterned motor activity (PMA) expressed by effector neurons of the buccal ganglion. Our analysis shows that a rise and fall of blood acidic amino acids corresponds to the suppression of feeding. Furthermore, bath application of glutamate at its elevated physiological level (100-150 microM) to buccal ganglia caused complete and prolonged suppression of PMA in effector neurons. This suppression is attributable to activation of chloride and potassium currents which shunt patterned synaptic inputs to the effector neurons. We conclude that glutamate, in an endocrine-like fashion, can exert a powerful neuromodulatory action on the feeding effector neurons of Helisoma.