MMP-3诱导人牙髓成纤维细胞CCN2mRNA的表达及意义 |
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引用本文: | 王贺,张娟,尹硕,李雯,王晓龙,唐宇欣,张颖丽. MMP-3诱导人牙髓成纤维细胞CCN2mRNA的表达及意义[J]. 口腔医学研究, 2014, 0(5): 399-402 |
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作者姓名: | 王贺 张娟 尹硕 李雯 王晓龙 唐宇欣 张颖丽 |
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作者单位: | 吉林大学口腔医院牙体牙髓病科 |
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基金项目: | 吉林省科技发展计划(编号:20130102096JC);长春市科技计划项目(编号:12SF88) |
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摘 要: | 目的:通过观察基质金属蛋白酶-3(MMP-3)对体外培养人牙髓成纤维细胞中结缔组织生长因子(CTGF/CCN2)mRNA的影响,揭示MMP-3促进牙髓损伤愈合的机制。方法:选取年轻患者因正畸或阻生拔除的健康第三磨牙,取出牙髓以常规体外酶消化培养法获得人牙髓成纤维细胞,利用RT-PCR方法检测经MMP-3诱导30min后人牙髓成纤维细胞中CCN2mRNA的表达。结果:正常组和实验组人牙髓成纤维细胞均有CCN2mRNA表达,但是经MMP-3诱导后的人牙髓成纤维细胞CCN2mRNA的表达更强。结论:MMP-3通过诱导CCN2mRNA的合成,从而促进牙髓损伤的愈合。
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关 键 词: | 人牙髓成纤维细胞 基质金属蛋白酶-3 结缔组织生长因子 |
Expression and Significance of CCN2 mRNA Induced by MMP-3 in Cultivate Human Pulp Fibroblasts |
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Affiliation: | WANG He;ZHANG Juan;YIN Shuo;Department of Endodontics,Stomatological Hospital of Jilin University; |
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Abstract: | Objective:To observe the effect of MMP-3on the mRNA of CCN2expression of cultivate human pulp fibroblasts in vitro and reveal the mechanism by which MMP-3promotes dental pulp wound healing.Methods:The human pulp fibroblasts were obtained from the healthy third molars which were extracted for orthodontic and impacted reason by tissue-explants collagenase digestion culture of pulp,The human pulp fibroblasts were stimulated by MMP-3.30minites later,the changes of CCN2mRNA expressed in human dental pulp fibroblasts were detected by means of RT-PCR.Results:CCN2mRNA expressed in both normal and experimental human pulp fibroblasts, but the expression of CCN2mRNA in human dental pulp fibroblasts which were induced by MMP-3was more significant.Conclusion:MMP-3which could induce the expression of CCN2mRNA in human pulp fibroblasts takes a critical part in dental pulp wound healing. |
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Keywords: | Human pulp fibroblasts MMP-3 CCN2 |
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