电化学发光法与酶法测定血清CK-MB含量与活性的比较

①目的比较电化学发光法测定血清肌酸激酶同工酶(CK-MB)含量与酶法测定CK-MB活性在急性心肌梗死(AMI)诊断中的可靠性.②方法应用电化学发光法与酶法分别测定了25例急诊AMI病人、20例非AMI病人和30例健康人的CK-MB含量和活性.③结果 AMI病人的CK-MB含量和活性到达峰值的时间相同,CK MB含量和活性AMI病人均高于非AMI病人和健康人,差异有显著性(F＝9.81,q＝10.58～15.09,P＜0.01);非AMI病人CK-MB活性亦高于健康人,差异有显著性(F＝5.07,q＝12.71,P＜0.05).④结论电化学发光法测定CK-MB含量是诊断心肌梗死的可靠指标.

DETECTION OF SERUM CK-MB CONTENT AND ITS ACTIVITY: A COMPARISION BETWEEN ELECTRO-CHEMILUMINESCENCE TECHNIQUE AND ENZYMOLOGY ASSAY

Objective To evaluate the reliability of serum creatine kinase isoenzyme(CK-MB) content determined by electrochemiluminescence (ECL) and its activity by enzymology assay in the diagnosis of acute myocardial infarction( AMI). Methods Serum CK-MB content and its activity were determined by ECL and enzymology respectively in 25 AMI patients, 20 non-AMI patients, and 30 healthy persons. Results CK-MB activity and content in serum of AMI patients reached peak at the same time, and were significantly higher than that of non-AMI patients and healthy persons, the difference was significant (F=9.81, q=10.58-15.09, P<0.01).The CK-MB activity in non-AMI patients was also significantly higher than that in healthy persons ( F=5.07,q=12.71,P<0.05). Conclusion Detection of serum CK-MB content by ECL is reliable in the diagnosis of AMI.