| 神经干细胞分化过程中NgR表达的变化 |
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| 引用本文： | 王丰,朱悦. 神经干细胞分化过程中NgR表达的变化[J]. 中国现代医学杂志, 2008, 18(16): 2291-2294 |
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| 作者姓名： | 王丰 朱悦 |
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| 作者单位： | 中国医科大学附属第一医院,骨科,辽宁,沈阳,110001 |
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| 基金项目： | 辽宁省自然科学基金，辽宁省教育厅资助项目 |
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| 摘 要： | 目的 检测大鼠脊髓来源神经干细胞分化过程中NgR的表达情况.方法 悬浮培养法培养大鼠脊髓来源的神经干细胞,形成神经球后,以免疫荧光法鉴定神经干细胞,检测神经球及其分化后不同时间点、不同细胞亚型的NgR表达情况.结果 神经干细胞分化前和分化第1天所有细胞都显著表达NgR,NgR表达阳性率在分化第2天降至82.1%±4.14%,分化第3天降至最低点35.3%±4.31%.分化后形成的细胞中只有神经元和少量未分化细胞表达NgR,星形胶质细胞和少突胶质细胞不表达NgR.结论 在神经干细胞分化成星形胶质细胞和少突胶质细胞的过程中NgR基因表达被关闭,而在其分化成神经元的过程中则始终表达NgR.
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| 关 键 词： | 神经干细胞 Nogo-66受体 |
Expression of NgR in neural stem cells changed during the differentiation progress |
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| WANG Feng,ZHU Yue. Expression of NgR in neural stem cells changed during the differentiation progress[J]. China Journal of Modern Medicine, 2008, 18(16): 2291-2294 |
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| Authors: | WANG Feng ZHU Yue |
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| Affiliation: | WANG Feng,ZHU Yue (Department of Orthopedics,the First Affiliated Hospital,China Medical University,Shenyang,Liaoning 110001,P.R.China) |
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| Abstract: | [Objective] To detect the expression of NgR (Nogo-66 Receptor) in neural stem cells (NSCs) derived from spinal cord of rats before and after their differentiation. [Methods] Neurospheres were collected by suspension culture of cells obtained from rat spinal cord. Immunofluorescence method was used to identify NSCs and detect expression of NgR before and after NSCs differentiated. [Results] All the NSCs express NgR significantly before differentiation and 1 d after differentiation. The NgR positive ratio dec... |
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| Keywords: | neural stem cells nogo-66 receptor |
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