| 神经干细胞分化过程中NgR表达的变化 |
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| 引用本文： | 王丰,朱悦.神经干细胞分化过程中NgR表达的变化[J].中国现代医学杂志,2008,18(16):2291-2294. |
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| 作者姓名： | 王丰 朱悦 |
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| 作者单位： | 中国医科大学附属第一医院,骨科,辽宁,沈阳,110001 |
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| 基金项目： | 辽宁省自然科学基金，辽宁省教育厅资助项目 |
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| 摘 要： | 目的 检测大鼠脊髓来源神经干细胞分化过程中NgR的表达情况.方法 悬浮培养法培养大鼠脊髓来源的神经干细胞,形成神经球后,以免疫荧光法鉴定神经干细胞,检测神经球及其分化后不同时间点、不同细胞亚型的NgR表达情况.结果 神经干细胞分化前和分化第1天所有细胞都显著表达NgR,NgR表达阳性率在分化第2天降至82.1%±4.14%,分化第3天降至最低点35.3%±4.31%.分化后形成的细胞中只有神经元和少量未分化细胞表达NgR,星形胶质细胞和少突胶质细胞不表达NgR.结论 在神经干细胞分化成星形胶质细胞和少突胶质细胞的过程中NgR基因表达被关闭,而在其分化成神经元的过程中则始终表达NgR.
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| 关 键 词： | 神经干细胞 Nogo-66受体 |
Expression of NgR in neural stem cells changed during the differentiation progress |
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| WANG Feng,ZHU Yue.Expression of NgR in neural stem cells changed during the differentiation progress[J].China Journal of Modern Medicine,2008,18(16):2291-2294. |
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| Authors: | WANG Feng ZHU Yue |
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| Institution: | WANG Feng,ZHU Yue (Department of Orthopedics,the First Affiliated Hospital,China Medical University,Shenyang,Liaoning 110001,P.R.China) |
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| Abstract: | Objective] To detect the expression of NgR (Nogo-66 Receptor) in neural stem cells (NSCs) derived from spinal cord of rats before and after their differentiation. Methods] Neurospheres were collected by suspension culture of cells obtained from rat spinal cord. Immunofluorescence method was used to identify NSCs and detect expression of NgR before and after NSCs differentiated. Results] All the NSCs express NgR significantly before differentiation and 1 d after differentiation. The NgR positive ratio dec... |
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| Keywords: | neural stem cells nogo-66 receptor |
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