Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay |
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| Authors: | Francois Patrice Koessler Thibaud Huyghe Antoine Harbarth Stephan Bento Manuela Lew Daniel Etienne Jérôme Pittet Didier Schrenzel Jacques |
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| Institution: | Genomic Research Laboratory, University of Geneva Hospitals, Service of Infectious Diseases/Genomic Research Laboratory, CH-1211 Geneva 14, Switzerland. patrice.francois@genomic.ch |
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| Abstract: | The accessory gene regulator (agr) is a crucial regulatory component of Staphylococcus aureus involved in the control of bacterial virulence factor expression. We developed a real-time multiplex quantitative PCR assay for the rapid determination of S. aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information. |
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