A novel circular invasion assay mimics <Emphasis Type="Italic">in vivo</Emphasis> invasive behavior of cancer cell lines and distinguishes single-cell motility <Emphasis Type="Italic">in vitro</Emphasis> |
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Authors: | Yoonseok Kam Cherise Guess Lourdes Estrada Brandy Weidow Vito Quaranta |
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Institution: | (1) Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN, USA;(2) Department of Microbiology, Meharry Medical College, Nashville, TN, USA |
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Abstract: | Background Classical in vitro wound-healing assays and other techniques designed to study cell migration and invasion have been used for many years to
elucidate the various mechanisms associated with metastasis. However, many of these methods are limited in their ability to
achieve reproducible, quantitative results that translate well in vivo. Such techniques are also commonly unable to elucidate single-cell motility mechanisms, an important factor to be considered
when studying dissemination. Therefore, we developed and applied a novel in vitro circular invasion assay (CIA) in order to bridge the translational gap between in vitro and in vivo findings, and to distinguish between different modes of invasion. |
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