IL—4对NOD小鼠1型糖尿病免疫干预及其机制的探讨

目的：探讨IL－4对NOD鼠1型糖尿病发病率,胰岛炎的影响及其机制。方法：采用人类1型糖尿病动物模型NO（nonobese diabetic)鼠,IL－4处理后检测血糖,尿糖及糠愕病发病率,HE染色观察胰岛炎程度,流式细胞仪测定周围及中枢淋巴细胞亚群的变化,结果IL－4处理组糖尿病发病率为12．50％,明显低于对照组（62．50％）（P＜0．05）,且胰岛炎的严重程度亦低于对照组;处理组周围单个核细胞（CD 4/CD 8及IL－2R＋细胞百分比增高,胸腺CD＋4,CD＋8细胞百分比增高而D＋4 CD＋8细胞百分比下降（P＜0．01）。结论 IL－4有降低NOD鼠1型糖尿病发病率及胰岛炎严重程度的作用,这种作用可能与IL－4影响了淋巴细胞亚群的分布从而纠正了免疫调节失衡有关。

The immunotherapy of type1 diabetes in NOD mice with IL-4 and the study of the possible mechanism

Objective To study the effectiveness of IL-4 on type 1 diabetes in NOD mice and it's possible mechanism.Methods 4-week-old NOD mice,as a kind of type 1 diabetes model,were treated with IL-4 to investigate the incidence of insulitis,and lymphocyte subsets were determined by FACS.Results The incidence of diabetes in the IL-4 group was 12.50% which was obviously less than 62.50% in the control group ( P <0.05),and the degree of insulitis in the IL-4 group was also less than that in the control group.The lymphocyte subset in the IL-4 treated group showed higher CD 4/CD 8 cells and percentage of IL-2R cells in spleen,and higher proportion of CD 4/CD 8 but decreased CD 4/CD 8 cells in thymus.Conclusion IL-4 can prevent NOD mice from developing type 1 diabetes,which may be related to the fact that IL-4 corrected the imbalance of immunoregulatory in NOD mice.