人牙本质涎蛋白对体外培养的人牙乳头间充质细胞增殖和碱性磷酸酶活性的影响

目的 :观察人牙本质涎蛋白对体外培养的人牙乳头间充质细胞增殖和碱性磷酸酶活性的影响。方法 :分对照组和实验组 ,对照组是转染不含目的基因的空白质粒 pcDNA3的培养上清 ,实验组是转染pcDNA3 -hDSP重组质粒的培养上清。取第 5代人牙乳头间充质细胞 ,接种于 96孔板 ,对照组 6孔 ,实验组 10孔 ,用MTT法检测hDSP对体外培养的人牙乳头间充质细胞增殖的影响 ,用碱性磷酸酶检测试剂盒检测hDSP对体外培养的人牙乳头间充质细胞碱性磷酸酶活性的影响。结果 :hDSP能明显抑制体外培养的人牙乳头间充质细胞增殖 (P <0 .0 1) ,但能促进细胞内和培养上清中碱性磷酸酶的分泌 (P <0 .0 1)。结论 :hDSP能促进人牙乳头间充质细胞的分化和矿化

The effect of hDSP on growth and alkaline phosphatase activity of cultured human dental papilla ectomesenchymal cells

AIM:To observe the effect of hDSP on growth and alkaline phosphatase of aotivity of cultured human dental papilla ectoectomesenchymal(DPE) cells in vitro .METHODS:Two groups were divided: the medium transfected by recombined plasmid pcDNA3-hDSP as experimental groups; the medium transfected only by pcDNA3 as control. DPE cells of passage 5 were seeded on 96-well plate, but only 6 for control, 10 for experiment were used. The effect of hDSP on growth and alkaline phosphatase activity of cultured DPE cells were examined by methabenzthiazuron (MTT) and enzyme chemical method separately.RESULTS:The results showed that the hDSP could significantly inhibit DPE cell proliferation( P <0.01)and stimulate ALPase activity( P <0.01).CONCLUSION:The results suggest that the hDSP may play an important role in odontoblast differentiation and mineralization.