滋阴益气活血通络法对糖尿病大鼠胰岛功能保护的实验研究

目的:观察滋阴益气活血通络法对糖尿病大鼠血浆胰岛素(FINS)、C肽及超氧化物歧化酶(SOD)、丙二醛(MDA)水平的影响,探讨其保护胰岛功能的作用机制。方法:将50只Wistar大鼠随机分为正常组、模型组、达美康组、中药高剂量组、中药低剂量组5组,链脲佐菌素(STZ)60mg/kg一次性腹腔注射,复制糖尿病大鼠模型。以实验大鼠血浆胰岛素(FINS)、C肽及超氧化物歧化酶(SOD)、丙二醛(MDA)为观察内容,采用达美康做对比观察。结果:滋阴益气活血通络法可以降低血糖(BG)和糖化血清蛋白(GSP),升高血浆胰岛素和C肽水平,与对照组结果相比无统计学意义(P>0.05),对大鼠SOD和MDA的影响则明显优于对照组(P<0.05),大、小剂量组中药对SOD和MDA的影响以大剂量组的疗效占优(P<0.01)。结论:滋阴益气活血通络法可能通过抗氧化而起到保护糖尿病大鼠胰岛B细胞功能的作用。

Experimental Study of Nourishing Yin,Invigorating Qi,Promoting Blood Circulation and Draining Collateral Method to DM Rats' Islet Protective Function

Objective:To observe the effect of nourishing Yin,invigorating Qi,promoting blood circulation and draining collateral method on levels of Insulin,C-peptide,SOD,and MDA in DM rats and to discuss the mechanism of the method to DM rats islet function.Methods:50 Wistar rats were randomly divided into 5 different groups: normal group,model group,gliclazide group,high dose TCM group,low dose TCM group.STZ 60mg/kg The DM rats models,compared with gliclazide,observed the levels of insulin,C-peptide,SOD and MDA in model rats.Results:Nourishing Yin,invigorating Qi,promoting blood circulation and draining collateral method can reduce the levels of BG and GSP,improve the levels of Insulin and C-peptide in model rats,Compared with control group,there were no statistics significance(P>0.05),the effect of SOD and MDA in DM rats was better than control group obviously(P<0.05),and the curative effect of the high dose TCM was better than the low dose TCM(P<0.01).Conclusions:Nourishing Yin,invigorating Qi,promoting blood circulation and draining collateral method protect the DM rats islet B cells function by antioxidation maybe.