| 应用液体蛋白芯片飞行时间质谱系统研究胃癌、肝癌血清差异蛋白表达 |
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| 引用本文： | 李莉,谌宏鸣,李甜,胡晓慧.应用液体蛋白芯片飞行时间质谱系统研究胃癌、肝癌血清差异蛋白表达[J].第四军医大学学报,2009,30(8):733-737. |
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| 作者姓名： | 李莉 谌宏鸣 李甜 胡晓慧 |
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| 作者单位： | 李莉,LI Li(新疆医科大学附属肿瘤医院,新疆,乌鲁木齐,830011);谌宏鸣,李甜,CHEN Hong-Ming,LI Tian(新疆医科大学基础学院组织胚胎学教研室,新疆,乌鲁木齐,830054);胡晓慧,HU Xiao-Hui(博扬通北京科技有限公司,北京,100080)
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| 基金项目： | 新疆维吾尔自治区高校科研计划重点项目 |
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| 摘 要： | 目的：研究胃癌、肝癌和正常人的血清差异蛋白表达,寻找具有潜在诊断意义的血清标志物,以利于胃癌、肝癌的早期诊断.方法：收集胃癌患者20例和肝癌患者10例以及正常人20例血清样本,经ClinProt磁珠纯化、基质辅助激光解析电离飞行时间质谱（MALDI-TOF-MS）分析及ClinPro-Tools生物信息学方法研究其血清蛋白表达谱.结果：质荷比（m/z）为2863u和4965u的蛋白峰能较好地鉴别胃癌和非癌组.m/z为1618u和4965u的蛋白峰能较好地鉴别肝癌和非癌组.m/z为4965u的蛋白峰在胃癌、肝癌中较正常人均异常高表达.结论：液体蛋白芯片飞行时间质谱系统作为研究蛋白表达谱的工具,具有较好的重复性与精度,筛选得到的m/z为2863,1618,4965u的蛋白可能为潜在的胃癌、肝癌肿瘤标志物.
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| 关 键 词： | 胃肿瘤 肝肿瘤 液体蛋白芯片飞行质谱 差异蛋白 早期诊断 |
Differential protein expression of gastric cancer and liver cancer in serum using magnetic bead based sample fractionation and MALDI-TOF-MS |
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| LI Li,CHEN Hong-Ming,LI Tian,HU Xiao-Hui.Differential protein expression of gastric cancer and liver cancer in serum using magnetic bead based sample fractionation and MALDI-TOF-MS[J].Journal of the Fourth Military Medical University,2009,30(8):733-737. |
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| Authors: | LI Li CHEN Hong-Ming LI Tian HU Xiao-Hui |
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| Institution: | LI Li1,CHEN Hong-Ming2,LI Tian2,HU Xiao-Hui31Department of Gynecology,Affiliated Tumor Hospital,Xinjiang Medical University,Urumqi 830011,China,2Department of Histology and Embryology,Urumqi 830054,3Beijing Bioyong Technologies Inc.,Beijing 100080 |
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| Abstract: | AIM:To establish differential proteintial expression using functional magnetic bead based sample fractionation method combined with matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS) technology and to explore potential biomarkers of gastric cancer and liver cancer for early diagnosis. METHODS:Plasma protein profiling of three groups including 20 gastric cancer patients and 10 liver cancer patients as well as 20 normal controls was analyzed by ClinProt magnetic bead en... |
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| Keywords: | gastric neoplasms liver neoplasms ClinProt system differential protein early diagnosis |
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