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回转器模拟失重对SGC-7901细胞增生和周期的影响
引用本文:朱鸣,吴本俨,聂捷琳,李莹辉. 回转器模拟失重对SGC-7901细胞增生和周期的影响[J]. 世界华人消化杂志, 2005, 13(7): 856-859
作者姓名:朱鸣  吴本俨  聂捷琳  李莹辉
作者单位:1. 解放军总医院南楼消化科,北京市,100853
2. 航天医院工程研究所第十三研究室,北京市,100094
基金项目:总装备部预研基金项目,No.51400020204JB1002~~
摘    要:

关 键 词:模拟失重  胃癌  细胞增生  细胞周期
修稿时间:2005-01-31

Effects of rotating clinostat simulated weightlessness on cell proliferation and cell cycle of human gastric carcinoma cell line SGC-790t
Ming Zhu,Ben-Yan Wu,Jie-Lin Nie,Ying-Hui Li. Effects of rotating clinostat simulated weightlessness on cell proliferation and cell cycle of human gastric carcinoma cell line SGC-790t[J]. World Chinese Journal of Digestology, 2005, 13(7): 856-859
Authors:Ming Zhu  Ben-Yan Wu  Jie-Lin Nie  Ying-Hui Li
Affiliation:Ming Zhu,Ben-Yan Wu,Department of Gastroenterology,South Building,the General Hospital of Chinese PLA,Beijing 100853,China Jie-Lin Nie,Ying-Hui Li,the 13th laboratory of Institute of Space Medico-Engineering,Beijing 100094,China
Abstract:AIM: To investigate the effect of simulated weightlessness on cell cycle and proliferation of human gastric carcinoma cell line SGC-7901. METHODS: A rotating clinostat was used to simulate weightlessness. The expression of PCNA was examined by immunohistochemical staining. The changes of cell cycle were examined by flow'cytometry. RESULTS: The expression of PCNA did not change significantly after 12 h, 24 h, and 36 h of simulated weightlessness, but decreased significantly after 48 h and 72 h (P<0.05 and P<0.01, respectively). The cell cycle of 12 h, 24 h, and 36 h groups did not change significantly. However, the cells in G0+G1 phase in 48 h and 72 h groups were increased significantly (P<0.01 and P<0.05, respectively), while the cells in S+G2+M phase were decreased significantly (P<0.01 and P<0.05). CONCLUSION: Simulated weightlessness by a clinostat can prevent the transition of cells from G1 phase to S phase, and thus the proliferation of SGC-7901 cells was inhibited.
Keywords:Simulated weightlessness  Gastric cancer  Cell proliferation  Cell cycle
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