食管鳞癌细胞转铁蛋白受体的免疫组化研究

本文应用转铁蛋白受体（ＴＦＲ）的单克隆抗体ＯｋＴ９，以后蜡切片ＡＢＣ法研究了６７例食管鳞癌、６６例不典型增生及２９例正常食管鳞状上皮（对照组）ＴＦＲ的表达。结果表明，鳞癌组ＴＦＲ染色强度显著高于不典型增生组（Ｐ＜０．００５）和对照组（Ｐ＜０．０２５）；鳞癌组和不典型增生组ＴＦＲ染色阳性率显著高于对照组（Ｐ＜０．００５）；对照组ＴＦＲ阳性染色仅见于基底层或基底层和棘细胞层，不典型增生组ＴＦＲ阳性染色见于上皮全层或中下层，鳞癌组ＴＦＲ阳性染色见于上皮内已癌变的细胞和侵入肌层的癌细胞。三组中，间质ＴＦＲ染色均阴性。由此提出，ＴＦＲ在三组染色强度和阳性率的差异，可能为应用ＴＦＲ动态监测鳞状上皮细胞癌变提供了新的指标；可能用于食管鳞癌的普查与筛选以及作为生物导向诊断与治疗的一种潜在靶抗原。

Immunohistochemical Study on the Transferrin Receptor of Squamous Cell Carcinoma of the Esophagus

The authors studied the transferrin receptor(TFR) in 67 squamous carcinomas of theesophagus,66 dysplastic epithelia and 29 normal epithelia (control group)by using a monoclonal antibody specific to TFR (OKTg) on paraffin sections. The results were as follows:The TFR staining intensity in the carcinoma group was significantly stronger than that inboth the dysplastic epithelium group and the control group (P<0. 005,P<0. 025). The TFRstaining positive rate in control group was significantly lower than in other two groups(PM0. 005). The TFR staining was found to be on the hasal layer or middle-lower layers in thecontrol group,on all the epithelial layers or middle-lower layers in the dysplastic epitheliumgroup and on the carcinoma cells within esophageal squamous epithelium or on the musclelayer in the carcinoma group. No TFR staining of mesenchyme was found in any of thegroups. The discrepancy of the TFR staining intensity and positive rate among the threegroups indicate that the TFR may be new marker for monitoring the canceration of theesophageal spuamous cells.