复方首乌对受损内皮促修复及释放PGI_2的作用

在体外培养内皮细胞单层上造成机械损伤（８．６４±１．１２）ｍｍ２。损伤后继续培养２４ｈ，收集上清液用放射免疫法测定其中６－酮－前列腺素Ｆ１α（ＰＧＦ１α）的含量，同时取出有内皮生长的玻片，染色后在光镜下观察内皮的生长情况。结果为：损伤组ＰＧＦ１α含量显著低于对照组，内皮修复现象不明显；损伤加药组（损伤内皮后，培养液内加入复方首乌液，继续培养２４ｈ），ＰＧＦ１α显著高于损伤组（Ｐ＜０．０１），损伤区两侧的内皮细胞开始修复损伤，表现为内皮细胞形成生长锥向损伤区生长，并出现明显的增殖现象，以上结果提示复方首乌液对受机械损伤的内皮有促进修复及合成并释放ＰＧＩ２的作用。

The Promoting Effects of Polygonum Multiflorum Mixture on Reendothelialization and PGI2 Release of Endothelial Cells Wounded in Vitro

Mechanical injury of (8.64±1.12)mm2 was made on monolayer of endothelial cells(EC) cultured in vitro. After injury a 24 hours’culture was followed. At the end of culture, the supernatant was collected for the PGF1α immunoradioassay. At the same time, EC was stained and observed under the light microscope. The results were as follows: The PGF1α content in group I (injury group) was significantly lower than that in the control group. The EC repair was not distinct. In group I+M (the group for which after the EC was denuded, the polygonum multiflorum mixture was added to the culture medium) the PGF1α content was higher than that in the group I significantly (P<0.01), the EC that survived beside the denuded area began to repair the denuded area by forming growth cone and increasing the amitotic figures. These results suggest that the polygonum multiflorum mixture is effective for promoting the reendothelialization and the PGI2 synthesis and relese of EC.