Characteristics of manganese (Mn) transport in rat brain endothelial (RBE4) cells, an in vitro model of the blood-brain barrier |
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Authors: | Fitsanakis Vanessa A Piccola Greg Aschner Judy L Aschner Michael |
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Affiliation: | Department of Pediatrics, B-3307 Medical Center North, Vanderbilt University School of Medicine, Nashville, TN 37232-2495, USA. |
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Abstract: | Manganese (Mn), an essential elemental nutrient, is known to be neurotoxic at high occupational levels. We examined the transport of Mn across a monolayer of rat brain endothelial cell (RBE4) to evaluate whether an electromotive permeability mechanism is responsible for Mn transport across the blood-brain barrier (BBB). The (54)Mn(2+) apparent permeability and flux showed significant temperature-, energy- and pH-dependence, as well as partial sodium-dependence. Additionally, iron (Fe)-rich and Fe-deficient media significantly increased the apparent permeability of (54)Mn(2+). Finally, Mn flux and permeability decreased when RBE4 cells were grown in astrocyte-conditioned media (ACM), compared to standard alpha-media. These data reinforce observations that transport of Mn across the BBB occurs in part through active transport process. |
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