银翘温胆汤干预大鼠放射性肺纤维化的初步实验研究

目的应用大鼠放射性肺纤维化模型观察银翘温胆汤对早期放射性肺炎和晚期肺纤维化的干预效果。探讨其在治疗放射性肺纤维化中的作用机制,以期为临床应用提供实验依据。方法将雄性大鼠45只随机分为对照组(0Gy+生理盐水)、照射组(20Gy+生理盐水)、银翘温胆汤组(20Gy+中药),单次6MV X线20Gy照射大鼠双肺,建立放射性肺纤维化模型。分别于2、4、6周时各处死5只大鼠取肺组织,进行HE染色观察肺组织病理变化,检测肺系数,用免疫组化法测转化生长因子-β1(TGF-β1)的表达。结果照射组大鼠肺损伤明显,肺系数、肺纤维化和TGF-β1的表达较对照组明显增高(P<0.05),而银翘温胆汤组大鼠比照射组的肺充血、出血、渗出性改变及肺泡壁增厚、肺纤维化程度减轻,且肺系数和TGF-β1的表达有一定程度的下降。结论银翘温胆汤对大鼠早期放射性肺炎和肺纤维化有一定程度的保护作用,其机制可能是通过抑制TGF-β1表达,使放射性肺炎及肺纤维化病变减轻。

Preliminary experimental study on Yinqiaowendantang in intervention of radiation pulmonary fibrosis in rats

Objective To investigate the intervention effects of Yinqiaowendantang on early stage radiation pneumonitis and advanced stage pulmonary fibrosis in rats. Methods Forty five male rats were randomly divided into the control group(0Gy+physiological saline),the irradiated group(20Gy+physiological saline) and the Yinqiaowendantang group(20Gy+traditional Chinese medicine).The chest of rats in irradiated group and the Yinqiaowendantang group was irradiated with a single dose 20Gy with 6MV-X ray,radiation pulmonary fibrosis model was built.On week 2,4,6 after treatment,5 rats of each group were sacrificed,and the lungs and blood were harvested for lung index measurement and pathological examination by HE stain,the expression of transforming growth factor-β1(TGF-β1) was measured by immunochemical method. Results Pathological study showed marked lung injury in irradiated group,the expression of lung index,pulmonary fibrosis and TGF-β1 in the irradiated lungs increased(P<0.05).Compared with that in the control group,while only slight pulmonary congestion,hemorrhage,exudative changes,alveolar wall thickening and pulmonary fibrosis in the lungs of the Yinqiaowendantang group,the expression of lung index and TGF-β1 decreased. Conclusion Yinqiaowendantang has protective effects on early stage radiation pneumonitis and pulmonary fibrosis,which might be associated with down-regulating the expression of TGF-β1 to reduce the pathological change degree of radiation pneumonitis and fibrosis.