EFFECT OF NICOTINE ON GROWTH AND EXPRESSION OF APOPTOSIS-RELATED GENES OF SMALL CELL LUNG CANCER （SCLC） CELL

Incidence and mortality of lung cancer haveincreased obviously for recent tens years,andespecially,the latter has taken in the first place insome cities,such as Beijing.Smoking is importantcause of lung cancer onset.Nicotine is a majorcomponent in tobacco and responsible for smokingaddiction,and its concentration in smoker's blood isfrom90nmol/L to1000nmol/L.It can not onlycontract blood vessel,elevate blood pressure,speedup heart rate and impact endothelial cell of bloodvessel,but also inhib…

Effect of nicotine on growth and expression of apoptosis-related genes of small cell lung cancer (SCLC) cell

Objective: To investigate the effect of nicotine on growth and apoptosis-related gene expression of human small lung cancer cell. Methods: NCI-H446 cell line was cultured in the presence of various concentrations (1∼1000 ng/ml) of nicotine for 48 h. MTT was applied to evaluate the effect of nicotine on the growth of NCL-H446, small cell lung carcinoma (SCLC) cell line. After NCI-H446 cells were treated with 100 ng/ml for 48 h, human apoptosis-related gene cDNA expression profile microarray was used to detect the expression of 451 apoptosis-related genes in NCL-H446 cell line. Results: Significant proliferation promotion of nicotine (1∼10 ng/ml) on NCI-LH446 cells were observed, but with decreased promotion effect with increased concentration of nicotine in culture. Growth inhibition rates for 1, 10, 100 and 1000 ng/ml nicotine were −91.0%, −41.8%, −40.0% and 27.3% respectively. Microarray detection showed that significantly different expressions were found in71 of 451 apoptosis-related genes. 38 apoptosis-promoting gene and 30 apoptosis-inhibiting genes were up-regulated significantly (cy3/cy5>2.0), and only 3 showed significant down-regulation (cy3/cy5<0.5). Conclusion: Nicotine may promote growth of human SCLC cell, and regulate expression of apoptosis-related genes. Biography: XIE Zuo-fu (1957–), male, associate professor, Fujian Provincial Tumor Hospital, majors in cell and molecular biology of tumor.