Effect of IL-1ra on human dental pulp cells and pulpal inflammation |
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Authors: | Lu H-X Xiao M-Z Niu Z-Y Guo X-M Zhao S-L Wang H-G Guo H-Y |
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Affiliation: | Department of Endodontics, College of Stomatology, Fourth Military Medical University, Xi'an, China. luhuaix@netease.com |
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Abstract: | AIM: This study was conducted to investigate the effect of interleukin-1 receptor antagonist (IL-1ra) on the LPS-induced interleukin-1beta (IL-1beta) synthesis in human dental pulp cells and to assess the role of IL-1ra in pulpal inflammation. METHODS: IL-1beta from human dental pulp cells (HDP) was measured by sandwich ELISA; IL-1ra expression in pulpal tissue was detected by immunohistochemical staining. RESULTS: Stimulation of HDP with increasing concentrations of FnLPS resulted in dose-dependent IL-1beta production. The addition of IL-1ra reduced FnLPS-induced IL-1beta synthesis in human dental pulp cells. Significant inhibition of the FnLPS-induced IL-1beta synthesis was observed when IL-1ra was added before treating with FnLPS for 60 min. Large numbers of IL-1ra positive neutrophils, plasmacytes, endothelial cells and lymphocytes were observed in inflamed pulp tissue. CONCLUSIONS: IL-1ra could reduce LPS-stimulated IL-1beta synthesis, suggesting that IL-1ra may play a role in pulpitis. |
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Keywords: | human dental pulp cells (HDP) interleukin-1β (IL-1β) interleukin-1 receptor antagonist (IL-1ra) lipopolysaccharide (LPS) |
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