| 高效液相色谱纯化抗肾综合征出血热病毒血凝素单克隆抗体及特性鉴定 |
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| 引用本文： | 薛小平,商澎,汪美先,陈镔复,王海涛. 高效液相色谱纯化抗肾综合征出血热病毒血凝素单克隆抗体及特性鉴定[J]. 细胞与分子免疫学杂志, 1991, 0(2) |
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| 作者姓名： | 薛小平 商澎 汪美先 陈镔复 王海涛 |
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| 作者单位： | 第四军医大学微生物学教研室，第四军医大学微中心实验室，第四军医大学微生物学教研室，第四军医大学微中心实验室，第四军医大学微生物学教研室 西安 710032，西安 710032 |
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| 摘 要： | 本文报告从BALB/c小鼠免疫腹水及该腹水经40％饱和硫酸铵盐析沉淀的γ球蛋白用高效液相色谱层析技木(HPLC)纯化、制备抗肾综合征出血热病毒血凝素单克隆抗体。HPLC一步法对IgG1的回收率为52％,纯度97.4％;HPLC二步法回收率为67％～73％,纯度98.1％;盐析法回收率为85％,纯度≥70％。间接免疫荧光法测定HPLC纯化样品(二步法)的比活性提高49～51倍,血凝抑制滴度为1:5120。
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| 关 键 词： | 高效液相色谱 抗体 单克隆 肾综合征出血热病毒 血凝素 |
Purification and Identification of Monoclonal Antibodies against Hemagglutinin of Hemorrhagic Fever with Renal Syndrome Virus by High Performance Liquid Chromatographic Method |
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| Xue Xiaoping,Shang Peng,Wang Meixian,Chen Binfu , Wang Haitao. Purification and Identification of Monoclonal Antibodies against Hemagglutinin of Hemorrhagic Fever with Renal Syndrome Virus by High Performance Liquid Chromatographic Method[J]. Chinese journal of cellular and molecular immunology, 1991, 0(2) |
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| Authors: | Xue Xiaoping Shang Peng Wang Meixian Chen Binfu & Wang Haitao |
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| Abstract: | Monoclonal antibody against HFRSV was purified by HPLC from ascites of immune mouse ( BALB/c ) and the McAb-r that pericipited with 40% saturate ammonium sulfate from the ascites, respectively. The recovery rate of IgG was 52% and purify was 97% in HPLC one-stage method. It was 6T%-73% and 98% in HPLC two-stage method, and it was 85% and 70% in precipition method. The specific activity of the sample purified by HPLC two-stage method was 49-51 times that was purified before. The titer of HIT was 1:5120. The titer of ELISA was 1:640 at 0.47mg/ml, |
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| Keywords: | high performance liquid chromatographic method antibody monoclonal hemagglutinin hemorrhagic fever with renal syndrome virus |
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