HB-H-8树脂吸附低密度脂蛋白的体外实验研究

目的 目前,用于降低血液中低密度脂蛋白(LDL)的吸附分离材料尚存在需要改进的地方.HB-H-8树脂在吸附LDL方面尚无应用,本研究对HB-H-8树脂的吸附性能进行了初步评价.方法 首先实验室制备HB-H-8树脂,经过处理后用其对LDL高于正常范围的患者血清标本进行体外动态和静态吸附实验.结果 体外动态吸附结果表明,HB-H-8树脂对LDL的吸附饱和时间为2h,最适吸附温度为恒温37℃,体外静态吸附结果显示HB-H-8树脂对LDL平均吸附率为63.2％,而对高密度脂蛋白(HDL)的平均吸附率为1.9％.结论 HB-H-8树脂对LDL具有良好的特异性吸附性能,有望开发为一种低密度脂蛋白的血液净化医用吸附材料.

In vitro experimental study on removing low density lipoprotein with HB-H-8 resin adsorption

Objective Currently, adsorption materials applied to remove low density lipoprotein （LDL） still remain defects. HB-H-8 resin has not been put in use in removing LDL. This study focused on primary evaluation of adsorption capability of HB-H-8 resin. Methods HB-H-8 resin was used in the adsorption of LDL of patient that was higher than the normal range of blood sample in dynamic test and static test in vitro. Results The time of adsorption saturation was two hours, and the optimum temperature was 37 ℃. The results of adsorption in vitro showed that the average adsorption rates of LDL and high density lipoprotein （HDL） were 63.2% and 1.9%, respectively. Conclusion HB-H-8 resin has LDL higher adsorption capability, thus made it to be a potential blood purification material for the removal of LDL.