新型大孔隙磷酸钙骨水泥作为骨组织工程支架的相关研究

目的 探讨新型大孔隙磷酸钙骨水泥(CPC)材料支架的细胞毒性和对细胞黏附、生长和增殖的影响.方法 通过添加甘露醇制孔剂和应用磷酸钠溶液作为CPC固化液的方法合成新型CPC材料.通过CCK8法检测细胞在新型CPC材料浸提液中的生长增殖情况；通过电子扫描电镜测试材料孔径和细胞在材料表面上黏附生长情况；应用力学三点弯曲实验测试新型CPC的生物力学性能.结果 新型CPC材料的孔径值达到(267.43±118.01)μm,孔隙率为(66.15±6.91)％.新型CPC材料的最大负荷、抗弯强度和坚韧度较传统CPC均增加了约1倍(P＜0.05).新型CPC材料浸提液与细胞共培养2、4、6、8d后CCK8法测试吸光度(OD)值与阴性对照组比较其差异无统计学意义(P＞0.05).结论 新型CPC材料具有强大的生物力学性能、大孔隙、高孔隙率和良好的生物相容性,有望成为理想的骨组织工程支架.

Related research on a novel macropores calcium phosphate cement as bone tissue engineering scaffold

Objective To investigate the cell toxicity of a novel macropores calcium phosphate cement （CPC） scaffold and its influence on cell adhesion, growth and proliferation. Methods A novel CPC material was synthesized by means of adding mannitol porogens and applying sodium solution as the cement liquid. The cell growth and proliferation in the novel CPC material extraction was observed by CCK8 assay. Scanning electron microscopy was used to observe hole diameter of the material, cell adhesion and growth in the material. The experiment of three point bending was used to test the biomeehanic performance of the CPC material. Results The novel CPC material reached hole diameter value of （267.43 ± 118.01） μm, mieroporosity of （66.15 ±6.91）%. Maximum load, flexural strength and toughness of the novel CPC material was increased about one time compared to the traditional CPC （P〈0.05）. CCK8 assay showed there were no significant difference of the light absorption value of cells in the CPC extraction in the 4th, 6th, 8th day compare to the control group （P〉0.05）. Conclusion The novel CPC material has the strong biomechanics performance, macropores, high microporosity and excellent biocompatibility, which is promising for ideal bone tissue engineering scaffold.