坎地沙坦对肝纤维化大鼠胰岛素样生长因子结合蛋白-2表达的影响

目的 研究血管紧张素Ⅱ1型受体拮抗剂(ARB)坎地沙坦对大鼠四氯化碳(CCl4)肝纤维化模型的疗效,观察坎地沙坦对肝纤维化大鼠胰岛素样生长因子结合蛋白-2(IGFBP-2)的影响.方法 制备CCl4诱导的大鼠肝纤维化模型同时应用坎地沙坦灌胃,共8周.留取各组的血和肝组织,通过HE及Masson染色观察各组肝纤维化的程度.采用SABC免疫组织化学方法检测大鼠肝组织中IGFBP-2的表达水平,酶联免疫吸附法(ELISA)测定血清中IGFBP-2的水平.结果 经CCl4诱导成功建立大鼠肝纤维化模型.随着肝纤维化程度的进展,IGFBP-2在肝组织中阳性表达增强(P<0.05);坎地沙坦治疗组IGFBP-2阳性表达较肝纤维化模型组均减弱(P<0.05).结论 坎地沙坦具有良好的抗肝纤维化作用,可能与IGFBP-2降低有关.

Expression of insulin-like growth factor binding protein-2 and its intervention by candesartan in experimental hepatic fibrosis

Objective To explore the protective effects of angiotensin Ⅱtype 1(AT1)receptor blocker candesartan in hepatic fibrosis induced by chronic carbon tetrachloride(CCl4)in rats and to observe the influence in liver for insulin-like growth factor binding protein-2(IGFBP-2).Methods Except rats in control group,all were given intraperitoneal injections of 40 % CCl4,and from the first day of the intraperitoneal injection,rats in treatment groups were given candesartan for 8 weeks by gastric gavage.The liver tissues were obtained from the control group,the model group and the candesartan treated group in different period respectively.Histopathological study of liver tissue was done with hematoxylin-eosin(HE)and Masson trichrome staining.The expression of IGFBP-2 in different group was measured by S-ABC immunohistochemistry,IGFBP-2 levels were determined by enzyme-linked immunosorbent assay(ELISA).Results Experimental rat hepatic fibrosis model induced by CCl4 was established.The expression of IGFBP-2 in liver tissue increased with the development of hepatic fibrosis(P<0.05),and candesartan decreased the expression IGFBP-2 in hepatic fibrosis in rats(P<0.05).Conclusion The expression of IGFBP-2 in liver tissue increases with the development of hepatic fibrosis,and candesartan decreases the expression IGFBP-2 in hepatic fibrosis in rats,which might inhibit the liver fibrosis.