基于叉指金电极和声表面波技术的细菌内毒素快速检测方法的研究

目的 建立一种可用于定量细菌内毒素浓度的快速检测方法.方法 通过在叉指金电极上固定γ-氨丙基三乙氧基硅烷(3-APTES)和戊二醛为载体的多粘菌素B(PMB),使其与细菌内毒素的生物活性物质脂多糖发生反应;检测装置采用基于声表面波技术的双路差分电路,2路输出振荡信号经过混频器进行差分,根据频移量的变化表征内毒素含量.结果 频移变化与内毒素含量在75～130 EU/ml范围内基本成线性关系.结论 与传统法相比,检测时间缩短到10 min,测试灵敏度达到13.8 kHz/(EU·ml-1),重复性达到90％以上.

Study of rapid endotoxin detection based on interdigital gold electrode and SAW

Objective To establish a method of rapid detection of bacteria endotoxin based on interdigital gold electrode and surface acoustic wave （SAW）. Methods With the carriers of 3-APTES and glutaral pentanedial, Polymyxin B （PMB） was immobilized on interdigital gold electrode, and then reacted with the bacteria endotoxin＇ s biological active material （lipopolysacchride, LPS） selectively. Double differential circuit based on SAW was employed to complete the detection. Two output oscillation signals were differentiated through mixer and the endotoxin content was described by frequency shift. Results The experimental results indicated that there was a liner relationship between the frequency shift and endotoxin content within the content range of 75-130 EU/ml. Conclusion Comparing with traditional method, the detection time is shortened to 10 minutes, the sensitivity is 13.8 KHz/（EU. mL-1）, and repeatability is above 90%.