光动力疗法对牙菌斑生物膜活性及结构的影响

目的 探讨光动力疗法(PDT)对4种主要致龋菌形成的混合菌菌斑生物膜活性及结构的影响.方法以变形链球菌、血链球菌、嗜酸性乳杆菌和黏性放线菌为实验菌株,建立牙菌斑生物膜模型.实验分为3组:PDT组,洗必泰处理组,生理盐水处理组.平板菌落计数观察牙菌斑生物膜活性,并运用激光共聚焦显微镜(CLSM)对处理后的牙菌斑生物膜进行...

Effects of photodynamic therapy on the activity and structure of the dental plaque biofilms

Objective To investigate the effects of photodynamic therapy（PDT） on the activity and structure of the dental plaque biofilm formed by the main cariogenic bacteria, Methods S.mutans, S.sangius, L. aeidophilus and A.viscosus were chosen as the experimental bacteria to establish dental plaque biofilm model. They were separated into three groups： group of PDT,group of chlorhexidine and group of normal saline. The influence of the dental plaque biofilms was observed according to plate counting of bacteria method. The changes in the structure of biofilms after PDT were analyzed by means of confocal laser scanning microscopy. Results Compared to normal saline group, chlorhexidine eariogenic bacteria （CFU/ml） in plaque biofilms significantly reduced （P〈0.05）, with the bactericidal rate 57.84%. After PDT treatment against artificial caries model, the number of cariogenic bacteria （CFU/ml） in plaque biofilms reduced more significantly （P〈0.05）, the bactericidal rate up to 94.92%, and the structure of dental plaque biofilms changed evidently. Conclusion PDT is an effective method in eliminate cariogenic bacteria of dental plaque biofilms and to destroy the integrity of dental plaque biofilms, and it is a promising method for elimination of dental plaque and prevention of caries.