定量ELISA法检测健康人血浆抗呼吸道合胞病毒IgG抗体的可行性研究

目的  确认定量ELISA法检测健康人血浆抗呼吸道合胞病毒（respiratory syncytial virus，RSV）IgG抗体的可行性。方法  采用定量ELISA法检测健康人血浆，对初检的抗-RSV阳性血浆（抗-RSV效价>11 U/ml）进行复检和中和试验。结果  检测3041份血浆，初检和复检的血浆抗-RSV阳性率分别为5.4%和4.7%。定量ELISA法的特异性和精密性均良好，抗-RSV阳性血浆的有效检出率为87.8%。结论  定量ELISA法可用于健康人血浆抗RSV IgG抗体的大规模筛检。

Feasibility study on a quantitative ELISA for detecting IgG antibody against respiratory syncytial virus in healthy human plasma

Objective  To identify feasibility of a quantitative ELISA for detecting IgG antibody against respiratory syncytial virus (RSV) in healthy human plasma.  Methods  The quantitative ELISA was used to detect  anti-RSA IgG in healthy human plasma, and anti-RSV positive plasmas (anti-RSV titer＞11 u/ml) obtained by initial detection were detected again by the quantitative ELISA, and a neutralization assay was carried out meanwhile. Results  3041 plasma samples were detected, and plasma anti-RSV positive rates of initial and repeat detection were 5.4% and 4.7%, respectively. The quantitative ELISA had good specificity and precision. The efficient  detection rate of anti-RSV positive plasma was 87.8%.  Conclusion  The quantitative ELISA can be applied to mass screening of anti-RSV IgG antibody in healthy human plasma.