缺血预适应对失血性休克大鼠的保护作用研究

目的目的观察缺血预适应对失血性休克大鼠的保护作用。方法采用失血性休克复苏大鼠,观察缺血预适应对大鼠存活、血管收缩反应、血流动力学平均动脉压（MAP）、左室收缩压（LVSP）、左室舒张末压（LVEDP）、左室压最大上升/下降速率（±dp/dtmax）、心率（HR）]、肝肾灌注和线粒体功能的影响。结果缺血预适应可延长失血性休克复苏大鼠存活时间,恢复血管对去甲肾上腺素（NE）和Ca2＋的反应性,恢复MAP、LVSP、LVEDP、±dp/dtmax、HR、NE升压效应,增加肝肾血流量、线粒体呼吸控制率和Na＋-K＋-三磷酸腺苷（ATP）酶活性（P〈0.01）。结论缺血预适应恢复血管收缩反应,改善血流动力学、血流灌注和线粒体功能,从而延长失血性休克复苏大鼠存活时间。

Beneficial effects of ischemia preconditioning on hemorrhagic shock rats

Objective To observe the beneficial effects of ischemia preconditioning on hemorrhagic shock rats.Methods Using hemorrhagic shock rats,the animal survival time,vascular reactivity and calcium sensitivity were evaluated.And hemodynamic parameters were observed after ischemia preconditioning and shock-resuscitation including mean arterial blood pressure(MAP),left intraventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),maximal change rate of left intraventricular pressure(±dp/dtmax),heart rate(HR),blood flow and mitochondrial function of liver and kidney.Results As compared with simple fluid resuscitation,ischemia preconditioning significantly prolonged the survival time and survival rate of shock-resuscitation rats,increased the vascular contraction to norepinephrine(NE) and Ca2+,restored the hemodynamic parameters including MAP,LVSP,LVEDP,+dp/dtmax,-dp/dtmax,HR and the pressor effect of NE,increased the blood flow,the mitochondrial respiration control ratio(RCR) and Na+-K+-ATP activity of liver and kidney(P<0.01).Conclusion Ischemia preconditioning prolonged the animal survival time of hemorrhagic shock rats through increasing the vascular reactivity and calcium sensitivity and improving hemodynamics,blood flow and mitochondrial function of vital organs.