马来酸桂哌齐特对脑缺血后炎症反应、轴突蛋白的表达及神经功能的影响

目的研究马来酸桂哌齐特(CM)对大鼠急性脑缺血/再灌注后对脑组织炎症反应、神经丝蛋白-200(NF-200)的表达及神经功能的影响。方法成年雄性SD大鼠48只,随机设为正常组、假手术组、缺血/再灌注2d及7d组、CM治疗2d组及7d组,分别在两个时间点对动物进行神经功能评分,并用ELISA法测定脑组织IL-1β、IL-6含量及免疫组化法测定缺血侧皮质NF-200的表达。结果脑缺血/再灌注后第2天,缺血/再灌注组及CM治疗组大鼠脑内IL-1β、IL-6的表达明显高于正常组和假手术组(P<0.01),而NF-200的表达明显降低(P<0.01),神经功能缺损明显(P<0.05);经CM治疗后,大鼠脑内IL-1β、IL-6的过度表达被明显抑制(P<0.01),NF-200表达明显增加(P<0.01),但是神经功能缺损较缺血/再灌注组无明显改善(P>0.05)。至缺血/再灌注后第7天,缺血/再灌注组各指标较第2天时明显改善(P<0.01),而CM治疗组各指标的改善较缺血/再灌注组更为明显(P<0.01)。结论 CM能减轻缺血再灌注大鼠脑内炎症因子IL-1β、IL-6的表达,改善局部细胞外环境,促进轴突的再生及大鼠神经功能的恢复。

Effects of cinepazide maleate on cerebral inflammation,neurite protein and neural function in rats with focal cerebral ischemia /reperfusion

Objective To study the effects of Cinepazide maleate(CM) on brain inflammtion,NF-200 expression and neural function in rats after acute cerebral ischemia.Methods Forty-eight adult male SD rats were randomly divided into control group,sham group,MCAO/reperfusion group(2 d),MCAO/reperfusion group(7 d),CM treatment group(2 d) and CM treatment group(7 d).IL-1β,IL-6 in brain tissue,cortex NF-200 expression and neural function were observed at 2,7 days respectively by ELISA,immunohistochemistry after ischemia.Results On 2 d after acute cerebral ischemia,IL-1β,IL-6 expression of rats in MCAO/reperfusion group and CM treatment group was obviously higher than that of the control group(P<0.01),while NF 200 expression reduced significantly(P<0.01),and nerve function defect worse(P<0.05).However,after CM treatment,rat brain IL-1β,IL-6 was significantly inhibited(P<0.01) and NF-200 increased(P<0.01) while neurological function score notimproved(P>0.05).Until 7 d after ischemia,each index value in MCAO/reperfusion group was improved obviously(P<0.01),while improvement in CM treatment group was more significant(P<0.01).Conclusion Cinepazide maleate could alleviates IL-1β,IL-6 in brain tissue,improves regeneration of axons and the neural function of rats after focal ischemia/reperfusion.