SD大鼠胆汁淤积性肝纤维化动物模型的建立与评价

目的 通过胆管结扎法建立胆汁淤积性肝纤维化动物模型,并对模型进行评价与探讨.方法 选用禁食12 h、能自由饮水的SD雄性大鼠,腹腔麻醉后,分离胆管,以丝线两处剪断1 cm结扎胆管,分别于0、2、4、6、8周测定大鼠体质量、肝脏质量、肝功能、肝纤维化酶谱,镜下观察肝脏组织学及超微结构的改变,并设立假手术组和对照组.结果 4~6周后,模型组大鼠体质量及肝脏质量较假手术组和对照组明显下降(P＜0.05),肝脏湿质量明显下降(P＜0.05),造模第2周即可见肝功能及肝纤维化酶谱较0周时明显升高(P＜0.05),病理切片可见肝组织结构明显破坏,大量炎细胞浸润、胶原纤维增生及假小叶形成.结论 胆汁

Establishment and evaluation of cholestatic liver fibrosis model induced by bile duct ligation in SD rat

Objective To establish and evaluate the cholestatic liver fibrosis model induced by bile duct ligation in SD rat.Methods The bile ducts of the rats were occluded by ligation.Serum AST,ALT,TB,HA and LN were determined in 0,2,4,6,8 weeks separately,as well as body weight,liver weight and the changes of the liver histology and ultrastructure.A sham operation and normal group were used as control.Results After 2 weeks,serum AST,ALT,TB,HA and LN levels of the model group were much higher than those of the control group.After 4-6 weeks,body weight and liver weight were decreased significantly,amount of inflammatory cells infiltration in damage liver structure,collagen fiber hyperplasia and false flocculus were formed.Conclusion The model of liver cholestatic fibrosis induced by bile duct ligation is an ideal study model of liver fibrosis,because it is model-constructing method is simple,short for processing cycle,stable and harmless to human and enviroment.