楷木与四叶参组合物对水貂呕吐模型的作用

目的 采用新型水貂呕吐模型研究楷木与四叶参提取物的组合物（楷组合物）对水貂呕吐模型的作用.方法 （1）雄性水貂30只随机分成5组：顺铂模型对照组、昂丹司琼组、楷组合物（楷木提取物与四叶参提取物按1∶1混合）小、中、大剂量组,各组分别给予生理盐水、昂丹司琼、楷组合物处理30分钟后给予腹腔注射顺铂,观察顺铂注射后6小时内水貂呕吐潜伏期和干呕、呕吐次数.（2）雄性水貂30只随机分成5组：1- phenylbiguanide hydrochloride（PBG）模型对照组、昂丹司琼组、楷组合物（楷木提取物与四叶参提取物按1∶1混合）小、中、大剂量组,各组分别给予生理盐水、昂丹司琼、楷组合物处理30分钟后给予腹腔注射PBG,观察PBG注射后6小时内水貂呕吐潜伏期和干呕、呕吐次数.（3）将顺铂预处理的水貂在用药24小时时处死,取其回肠组织用高效液相电化学检测法（HPLC- ECD）测定5- 羟色胺（5- HT）含量,并观察楷组合物对顺铂引起的水貂回肠5- HT释放的影响.结果 楷组合物对顺铂和PBG所致水貂呕吐行为有抑制作用,可延长呕吐潜伏期、减少干呕和呕吐次数（P＜0.05）.水貂回肠HPLC结果示,顺铂可促进回肠5- HT释放,升高回肠内5- HT含量（P＜0.01）.楷组合物对顺铂引起的水貂回肠5- HT释放有抑制作用,可降低其含量的升高（P＜0.05）.结论 楷组合物有止呕作用;对顺铂引起的水貂回肠5- HT释放有抑制作用,可降低其含量的升高;止呕机制涉及到外周和中枢的5- HT及5- 羟色胺3（5- HT3）受体系统.

Effect of composition of Codonopsis lanceolata Pistacia and Pistacia chinensis Bge on vomiting model of mink.

Objective To investigate the antiemetic effect of the composition of Pistacia chinensis and Codonopsis lanceolata Benth. et Hook （ Jie Combination ） on acute emesis in the new vomiting model of minks. Methods （1） Minks were randomly divided into cisplatin group, cisplatin ＋ ondansetron group, cisplatin ＋ low-dose Combination group, cisplatin ＋ middle-dose Combination group and cisplatin ＋ high-dose Combination group. The antiemetie effect of drugs was investigated in the vomiting model of minks induced by cisplatin （7.5mg · kg^-1 , i. p. ） in acute period （6 h） observation. （2） Minks were randomly divided into 1-phenylbiguanide hydrochloride （PBC） group, PBG ＋ ondansetron group, PBG ＋ low-dose Combination group , PBG ＋ middle-dose Combination group and PBG ＋ high-dose Combination group. The antiemetic effect of drugs was investigated in the vomiting model of minks induced by PBG （ 8 mg· kg^-1, i. p. ） in acute period （6 h） observation. （3） Cisplatin pretreatment of mink in 24 h, detected the content of 5- HT of the intestinal tissue by high performance liquid chromatography with electrochemical detection （HPLC-ECD） , and observe the effect of Jie composition on 5-HT releasing on mink ileum. Resuits The frequency of retching and vomiting was significantly reduced by pretreatment with Jie Combina- tion in middle and high dose （ P 〈 0. 05 ） . Jie composition inhibited the release of 5- HT on cisplatin induced mink ileal （ P 〈 0. 05 ）. Conclusion Jie Combination has a good activity against acute emesis in minks , the mechanism may involve 5-HT and 5-HT3 receptors of peripheral and central.