RT-PCR法分析两种解表法中药对FM1感染小鼠肺中炎性细胞因子的影响

Influence of the lung inflammatory cytokines in model rats infected with influenza virus FM1 by treating with two kinds of Chinese medicine compounds for reliving exterior syndromes through RT-PCR method

Objective To observe the influence of the lung inflammatory cytokines expression in model rats infected with influenza virus FM1 by treating with two kinds of Chinese medicine compounds for reliving exterior syndromes （Including relieving exterior with warm-pungent herbs and cold-pungent herbs in Chinese medicine theories）. To discuss the inflammation damage and repairing mechanism of these two compounds. Methods Model rats infected with influenza virus FM1 were divided into blank group, model control group, warm-pungent herbs group （ consists of Ephedra, Notopterygium and Purple Perilla） and cold-pungent herb group （consists of Radix Bupleuri, Honeysuckle and Mentha Haplocalyx） at random. Each group was divided by the different stages of infection in 1, 3, 5 and 7 day（8 for each）. Then observe the influence of TNF-ot, IL-6, IL-1, IL-10 and IFN-γ in model rats lungs in each group. Results The RNA expression in model rat lungs of TNF-α in 1 - 5 day levels, IL-1 in 3 - 5 day levels and IL-6 in 1,7 day levels were significantly reduced in warm-pungent herb group, while the expression of IL-10 in 3, 7 day levels and IFN-γ in 1 - 3 day levels were significantly increased. In cold-pungent herb group, the expression of TNF-α in 3 - 5 day levels, IL-1 in 7 day level and IL-6 in 1 - 7 day levels were significantly reduced, while the expression of IL-10 in 3,7 day levels and IFN-γ in 1 - 3 day levels were significantly increased, but the expression of IL-1 in 3 day level was significantly increased. Conelusion Diaphoresis Chinese medicine compounds for reliving exterior syndromes based on those two relieving exterior methods might be able to decrease inflammatory damages and enhance the recovery of injured by correcting inflam- matory cytokine imbalance.