| 上海地区女性生殖道人乳头瘤病毒感染的检测 |
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| 引用本文： | 潘銮风,曹式芳,丁爱华. 上海地区女性生殖道人乳头瘤病毒感染的检测[J]. 复旦学报(医学版), 1992, 0(4) |
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| 作者姓名： | 潘銮风 曹式芳 丁爱华 |
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| 作者单位： | 上海医科大学基础医学部生物物理学教研室(潘銮风，曹式芳)，妇产科医院(丁爱华) |
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| 摘 要： | 应用地高辛甙元标记人乳头瘤病毒(HPV)-6,11,16和18探针,通过核酸杂交法检测上海地区女性患者HPV的感染。计活检组织标本128例,阴道脱落细胞130例,同时取样34例。斑点杂交结果表明活检组织中四种类型HPV的总阳性率:宫颈癌44％,宫颈间变66.7％,有间变史28.6％,外阴尖锐湿疣63％和慢性宫颈炎5.6％。阴道脱落细胞的各阳性率与之相近,同时取样的检测符合率为91.2％。Southern转移杂交结果提示:宫颈癌以HPV-16型为主,尖锐湿疣中的HPV为6和11型
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| 关 键 词： | 人乳头瘤病毒 女性生殖道 地高辛甙元 核酸杂交法 |
DETECTION OF HUMAN PAPILLOMAVIRUS INFECTION IN GENITAL OF FEMALE PATIENTS OF SHANGHAI AREA |
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| Pan Luanfeng,Oao Shifang,Ding Aihua. DETECTION OF HUMAN PAPILLOMAVIRUS INFECTION IN GENITAL OF FEMALE PATIENTS OF SHANGHAI AREA[J]. Fudan University Journal of Medical Sciences, 1992, 0(4) |
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| Authors: | Pan Luanfeng Oao Shifang Ding Aihua |
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| Abstract: | Detection of human papillomavirus infection in female patients of the Shanghai area by nucleic acid hybridization uaing digoxigenin labelled HPV-6, 11, 16 and 18 probes was performed on 128 biopsy, 129 exfoliated vaginal cell and 34 both biopsy and exfoliated cell specimens. Positive rates combined HPV-6, 11, 16 and 18 in biopsy specimens of oervicrl carcinoma, cervical dysplasia, past cervical dysplasia, vaginal condylomata and cervitis were 44%, 66.7%, 28.6 %, 63% and 8.6% respectively by spot hybridization. Similar results were obtained on exfoliated cell specimens. In an analysis of 34 patients with both biopsy and exfoliated cell the coincident detection rate was 91.2%. Southern blot hybridization results showed that HPV in cervical carcinoma was mainly type 16, whereas in vaginal condylomata, types 6 and 11. |
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| Keywords: | human papillomavirus genital digoxigenin nucleic acid hybridization |
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