| 肝癌患者粘附性LAK细胞制备及抗瘤活性研究 |
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| 引用本文： | 柴晓杰,叶胜龙,吴伟忠,孙瑞霞,贺平,汤钊猷. 肝癌患者粘附性LAK细胞制备及抗瘤活性研究[J]. 中国肿瘤生物治疗杂志, 1995, 2(3): 191-193 |
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| 作者姓名： | 柴晓杰 叶胜龙 吴伟忠 孙瑞霞 贺平 汤钊猷 |
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| 作者单位： | 上海医科大学肝癌研究所!上海200032 |
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| 基金项目： | 美国中华医学基金会资助课题(93-583) |
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| 摘 要： | 本研究在常规LAK细胞制备基础上进行改进，用苯丙氨酸甲酯(PME)去除肝癌患者外周血中抑制LAK细胞活性的单核巨噬细胞。制备抗癌活性较高扩增迅速的粘附性LAK细胞，从实验结果分析。外周血处理后粘附性LAK细胞较未粘性LAK细胞具有较强的扩增能力，最大扩增倍数23～243倍，同时粘附性LAK细胞中TH细胞亚群有增加趋势，Ts细胞亚群有碱少趋势，其IL-2R^ 表达增加至64．3%。此外,在抗瘤活性方面粘附性LAK细胞与非粘附性LAK细胞存在一定差别，分别为64．6%和42．8%．因此。从临床实用出发。制备相对高效的粘附性LAK细胞可以提高LAK疗法的治疗效果。
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| 关 键 词： | LAK细胞 肝癌患者 抗瘤活性 扩增 外周血 粘附性 细胞亚群 制备 甲酯 研究 |
| 收稿时间： | 1994-12-08 |
Preparation and Anti-tumor Activity of Adherent LAK Cells from Patients with Hepatocellular Carcinoma |
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| Chai Xiaojie,Ye Shenglong,Wu Weizhong,Sun Ruixi,He Ping and Tang Zhaoyou. Preparation and Anti-tumor Activity of Adherent LAK Cells from Patients with Hepatocellular Carcinoma[J]. Chinses Journal of Cancer Biotherapy, 1995, 2(3): 191-193 |
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| Authors: | Chai Xiaojie Ye Shenglong Wu Weizhong Sun Ruixi He Ping Tang Zhaoyou |
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| Affiliation: | Liver Cancer Institute, Shanghai Medical University. Shanghai 200032;Liver Cancer Institute, Shanghai Medical University. Shanghai 200032;Liver Cancer Institute, Shanghai Medical University. Shanghai 200032;Liver Cancer Institute, Shanghai Medical University. Shanghai 200032;Liver Cancer Institute, Shanghai Medical University. Shanghai 200032;Liver Cancer Institute, Shanghai Medical University. Shanghai 200032 |
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| Abstract: | Highly active adherent LAK cells (A-LAK) with monocytes depleted by phenylalahine methyl ester (PME) were cultured from peripheral blood lymphocytes of patients with hepatocellular carcinoma (HCC). Results showed that A-LAK cells cultured in about 14 days |
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| Keywords: | adherent LAK cells (A-LAK) phenylalanine methyl ester (PME) hepatocellular carcinoma (HCC) |
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