| 罗汉果甜苷对力竭运动大鼠胃组织HSP70mRNA及蛋白表达的影响 |
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| 引用本文： | 莫伟彬,卜凯,黄丽丽,刘婷,黄东,宫明明.罗汉果甜苷对力竭运动大鼠胃组织HSP70mRNA及蛋白表达的影响[J].中国实验方剂学杂志,2015,21(7):103-106. |
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| 作者姓名： | 莫伟彬 卜凯 黄丽丽 刘婷 黄东 宫明明 |
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| 作者单位： | 广西师范大学 体育学院, 广西 桂林 541004;药用资源化学与药物分子工程教育部重点实验室, 广西 桂林 541004,广西师范大学 体育学院, 广西 桂林 541004,广西师范大学 体育学院, 广西 桂林 541004,广西师范大学 体育学院, 广西 桂林 541004,广西师范大学 体育学院, 广西 桂林 541004,贺州学院 体育学院, 广西 贺州 542800 |
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| 基金项目： | 国家自然科学基金项目(31060121);广西高校科研项目(YB2014384);广西教育厅科研项目(2013YB030);"药用资源化学与药物分子工程教育部重点实验室"开放课题 (CMEMR2012-B04) |
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| 摘 要： | 目的:研究运动前补充罗汉果甜苷(Mog)对力竭运动大鼠胃组织热休克蛋白70(HSP70)mRNA表达和HSP70蛋白表达的影响,以初步探讨罗汉果甜苷的作用机制。方法:SD雄性大鼠50只,随机分为5组,每组10只,分别为安静组(NC),运动组(ME),运动+低剂量罗汉果甜苷组(Mog L),运动+中剂量罗汉果甜苷组(Mog M),运动+高剂量罗汉果甜苷组(Mog H)。每天在运动前30 min ig 1次,连续ig 6周,6 d/周,低、中、高3组的ig剂量分别为100,200,400 mg·kg-1,对照组ig等量生理盐水。6周力竭训练结束后,宰杀大鼠。根据试剂盒的方法测定大鼠胃组织HSP70mRNA及蛋白表达。结果:运动组大鼠胃组织HSP70mRNA表达和HSP70蛋白表达水平高于安静组(P0.05);Mog L,Mog M,Mog H各组大鼠胃组织HSP70mRNA表达和HSP70蛋白水平都高于安静组和运动组(P0.05,P0.01)。结论:罗汉果甜苷能够提高大鼠胃组织HSP70mRNA表达和HSP70蛋白表达水平,对减少胃组织的溃疡、促进损伤后的恢复、细胞生长和发育等方面发挥重要的作用。
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| 关 键 词： | 罗汉果甜苷 力竭运动 热休克蛋白70 表达 |
| 收稿时间： | 9/4/2014 12:00:00 AM |
Effects of Mogroside on Expression of HSP70 mRNA and Protein in Gastric Tissue of Exhaustive Rats |
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| MO Wei-bin,BU Kai,HUANG Li-li,LIU Ting,HUANG Dong and GONG Ming-ming.Effects of Mogroside on Expression of HSP70 mRNA and Protein in Gastric Tissue of Exhaustive Rats[J].China Journal of Experimental Traditional Medical Formulae,2015,21(7):103-106. |
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| Authors: | MO Wei-bin BU Kai HUANG Li-li LIU Ting HUANG Dong and GONG Ming-ming |
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| Institution: | Sport School of Guangxi Normal University, Guilin 541004, China;State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China, Guilin 541004, China,Sport School of Guangxi Normal University, Guilin 541004, China,Sport School of Guangxi Normal University, Guilin 541004, China,Sport School of Guangxi Normal University, Guilin 541004, China,Sport School of Guangxi Normal University, Guilin 541004, China and School of Physical Education, Hezhou University, Hezhou 542800, China |
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| Abstract: | Objective: Study on the expression of gastric tissue heat shock protein (HSP70) and HSP70 mRNA after exhaustive exercise in rats which pre-exercise supplement of mogroside(Mog), to preliminary research the mechanism of mogroside. Method: Fifty healty male Sprague-Dawley rats were randomized into five groups, each consisting of 10 rats:the quiet group (NC), the exercise group (ME), the administration of Mog in low dose exercising group (MogL), the administration of Mog in middle dose exercising group (MogM) and the administration of Mog in high dose exercising group (MogH). The rats were administrated by professional gavage once a day half an hour before exercising. This administration was continuously gaved six days in one week, and lasted for six weeks. The low, middle and high doses are respectively 100, 200, 400 mg·kg-1, while the rats in control group were administrated of pure physiological saline at the same dose. The rats were killed after 6-week training. The expression of gastric tissue protein HSP70 and HSP70 mRNA were measured according the corresponding kit method respectively. Result: The expression of gastric tissue protein HSP70 and HSP70mRNA were higher in exercise control group than these in NC group (P<0.05). The expression of gastric tissue protein HSP70 and HSP70 mRNA in MogL, MogM and MogH were higher than these in NC group and ME group (P<0.05, P<0.01). Conclusion: Supplement of Mog could improve the expression of gastric tissue protein HSP70 and HSP70 mRNA. This could play an important role to reduce gastric ulcer, promote recovery, growth of cell and development after injury. |
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| Keywords: | mogroside exhaustive exercise heat shock protein expression |
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