乌梅丸拆方对UC大鼠结肠上皮细胞Notch信号通路的影响

目的:观察乌梅丸拆方对溃疡性结肠炎大鼠结肠上皮细胞Notch信号通路的影响,揭示方中治疗溃疡性结肠炎的主要治法和药物。方法:以三硝基甲苯磺酸/乙醇灌肠建立大鼠溃疡性结肠炎模型。将80只大鼠随机分成8组:空白组(生理盐水10 m L·kg-1),模型组(生理盐水10 m L·kg-1),乌梅丸全方组(53.2 g·kg-1),寒热并用组(36.0 g·kg-1),温热药物组(21.32 g·kg-1),寒凉药物组(14.68 g·kg-1),补益药物组(6.68 g·kg-1),收敛药物组(10.68 g·kg-1),造模8 h后各组大鼠按剂量灌胃给药,给药10 d。取结肠组织做病理学检查并采用实时荧光定量核酸扩增检测系统(Q-PCR)法检测结肠上皮细胞Notch-1,Hes-1,Math-1 mRNA的表达。结果:与空白组比较,模型组大鼠结肠上皮细胞Notch-1,Hes-1 mRNA表达显著增强,Math-1 mRNA表达降低(P0.05);与模型组比较,乌梅丸及各拆方给药后,乌梅丸组,寒热并用组大鼠结肠上皮细胞Notch-1,Hes-1 mRNA表达明显降低,Math-1 mRNA表达显著增高(P0.05)。结论:乌梅丸方中对Notch信号通路有调节作用的主要是寒热并用组药物,寒热并用法是该方治疗溃疡性结肠炎的主要治法。

Effect of Wumei Pill and Its Subdivisions on Notch Signal Pathway in Colonic Epithelial Cells of Ulcerative Colitis in Rats

Objective: To observe the effect of Wumei pill and its subdivisions on Notch signal pathway in colonic epithelial cells of ulcerative colitis(UC) rats, and explore the main therapy and drug of traditional Chinese medicine. Method: The ulcerative colitis model was established by giving 2, 4, 6-three nitro toluene sulfonate(TNBS)/ethanol enemas. Eighty rats were randomly divided into 8 groups : blank group(physiological saline 10 mL·kg^-1), model group ( physiological saline 10 mL·kg^-1), Wumei pill group(53.2 g·kg^-1), cold and heat application group(36.0 g·kg^-1), heat group(21.32 g·kg^-1), cold group(14.68 g·kg^-1), tonic group(6.68 g·kg^-1)and astringent group(10.68 g·kg^-1). After 8 hours, all the groups were given Wumei pill or its subdivisions once a day. After 10 days, the colon tissue was collected for pathology examination and the Notch-1, Hes-1, Math-1 mRNA expressions of colonic epithelial cells of rats were tested by Real-time quantitative PCR detecting system(Q-PCR) method. Result: Compared with blank group, Notch-1, Hes-1 mRNA expression was significantly enhanced while Math-1 mRNA was decreased in model group rats(P<0.05). Notch-1, Hes-1 mRNA expression was significantly decreased while Math-1 mRNA was enhanced in Wumei pill group and cold and heat application group(P<0.05). Conclusion: The cold and heat application group play an important role in regulating the Notch signal pathway in Wumei pill, and the therapy of cold and heat is the main method for treatment of ulcerative colitis.