DNA甲基转移酶在肝细胞癌中的表达及其临床意义

目的:探讨DNA甲基转移酶1(DNMT1)、DNA甲基转移酶3a(DNMT3a)和甲基转移酶3b(DNMT3b)在肝细胞癌(HCC)中的表达及其意义.方法:应用免疫组化检测47例HCC癌组织、42例HCC癌旁肝组织及12例正常肝组织中DNMT1、DNMT3a和DNMT3b的表达情况,分析三者与临床病理特征之间的关系.结果:DNMT1、DNMT3a和DNMT3b在HCC中阳性表达分别为80.9%、68.1%和78.7%,在癌旁组织内的表达分别为50.0%、52.4%和57.1%,均明显高于正常肝组织内的阳性表达(16.7%、16.7%和25.0%),且DNMT1、DNMT3a和DNMT3b与HCC的病理分化类型及乙肝表面抗原阳性显著相关(P<0.05).结论:DNMT1、DNMT3a和DNMT3b的异常表达与HCC的发生发展有紧密的关系.

Expression and clinical significance of DNA methyltransferases in Hepatocellular Carcinoma

Objective To investigate the expression and significance of DNA methyltransferase 1(DNMT1),3a(DNMT3a) and 3b(DNMT3b) in human hepatocellular carcinoma(HCC).Methods The expression of DN MT1,DNMT3a and DNMT3b was detected in 47 cases of HCC tumor specimen and 42 cases of HCC paracarci noma liver tissue by immunohistochemistry,12 cases of normal liver tissue as control;the results and clinical pathological parameters were analyzed.Results The positive expression rate of DNMT1,DNMT3a and DN MT3b in HCC tissues was 80.9%、68.1% and 78.7% respectively.The positive expression rate of DNMT1,DN MT3a and DNMT3b in paracarcinoma tissues was 50.0%、52.4%and 57.1% respectively.All of them are significantly higher than that in normal liver tissues(16.7%、16.7%和25.0%).The expression of DNMT1,DNMT3a and DNMT3b was correlated with tumor differentiationand and hepatitis B surface antigen(HBsAg)(P < 0.05).Conclusion DNMT1,DNMT3a and DNMT3b maybe play important roles on carcinogenesis and the develop ment of HCC.