| 云南省输入性登革热病例登革病毒1型的分离研究 |
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| 引用本文： | 郭晓芳,李鸿斌,卢云兰,杨中华,朱进,周红宁. 云南省输入性登革热病例登革病毒1型的分离研究[J]. 中国病原生物学杂志, 2012, 0(4): 241-243 |
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| 作者姓名： | 郭晓芳 李鸿斌 卢云兰 杨中华 朱进 周红宁 |
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| 作者单位： | 云南省寄生虫病防治所;西双版纳州疾控中心;大理学院病原与媒介生物研究所(普洱分部) |
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| 基金项目： | 国家自然科学基金项目(No.30960327) |
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| 摘 要： | 目的对2011年8月采集的从老挝输入的登革热病人急性期血清进行登革病毒分离,以找出病原学证据。方法用C6/36白纹伊蚊细胞进行病毒分离,对有细胞病变的标本进行RT-PCR,检测登革病毒RNA及型别,对PCR产物测序,进行比对分析和进化树分析。结果从8例输入登革热病人的血清中分离到4株能引起细胞病变的病毒,均为登革病毒1型,与泰国毒株的同源性99%。结论云南省首次从输入登革热病人血清中分离到登革病毒1型,为云南省登革热的防控提供了依据。
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| 关 键 词： | 登革热 登革病毒1型 输入病例 云南省 |
The first report of isolation of dengue virus type 1 from sera of patients with imported dengue in Yunnan Province |
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| GUO Xiao-fang,LI Hong-bin,LU Yun-lan,YANG Zhong-hua,ZHU Jin,ZHOU Hong-ning. The first report of isolation of dengue virus type 1 from sera of patients with imported dengue in Yunnan Province[J]. Journal of Pathogen Biology, 2012, 0(4): 241-243 |
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| Authors: | GUO Xiao-fang LI Hong-bin LU Yun-lan YANG Zhong-hua ZHU Jin ZHOU Hong-ning |
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| Affiliation: | 1(1.Yunnan Institute of Parasitic Diseases,Pu’er,Yunnan 665000,China;2.The Centers for Disease Control and Prevention of Xishuangbanna,Jinghong,Yunnan 666100,China;3.Institute of Pathogens and Vectors,Dali University,Pu’er,Yunnan 665000,China) |
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| Abstract: | Objective To confirm dengue infection and type the dengue virus in cases of dengue imported to Yunnan Province from Lao PDR in 2011.Methods Isolation of the dengue virus was done using C6/36 cells.Dengue nucleic acid was detected in the isolates via a stable cytopathic effect on C6/36 cells according to RT-PCR.The dengue virus E gene was sequenced and analyzed using BLAST.Phylogenetic analyses based on the E gene of new viral isolates were conducted using MEGA4.Results Four dengue virus isolates were obtained from eight serum samples.All were identified as dengue virus type 1.The nucleotide homology with dengue virus type 1 isolated from Thailand was greater than 90%.Conclusion This is the first isolate of dengue virus type 1 from dengue cases imported to Yunnan Province from Lao PDR.These results provide scientific evidence for dengue control in Yunnan Province. |
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| Keywords: | Dengue fever dengue virus type 1 imported cases Yunnan Province,China |
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