稳心颗粒对异丙肾上腺素诱发豚鼠心律失常的电生理影响

目的探讨稳心颗粒对异丙肾上腺素(Iso)诱发心律失常的电生理影响。方法用标准微电极细胞内记录技术,观测稳心颗粒对异丙肾上腺素诱发豚鼠心律失常的影响。观测指标:最大舒张电位(maxi mal diastolic potential,MDP)、动作电位幅度(am-plitude of action potential,APA)、0相最大去极速度(maxi mal rate of depolarization,Vmax)、4相自动去极速度(velocity of diastolicdepolarization,VDD)、复极50%和90%时间(50%and90%of duration of action potential,APD50and APD90)以及自发放电频率(rate of pacemaker firing,RPF)。结果用100μmol/LIso灌流后左心室流出道自律细胞出现了心律失常,用Iso+稳心颗粒灌胃组血浆灌流后,与Iso模型组和Iso+生理盐水灌胃血浆比较,RPF和VDD减慢(P0.01),APA和MDP的绝对值显著减小(P0.01),Vmax明显减慢(P0.01),APD80和APD90明显延长(P0.01)。结论稳心颗粒能降低自发放电频率,拮抗异丙肾上腺素诱发的心律失常。

The Electrophysiological Effects of Wenxin Granule on Arrhythmia Induced by Isoprenaline in Guinea Pig

Objective To explore the electrophysiological effects of Wenxin granule on arrhythmia induced by isoprenaline in guinea pig.Methods Arrhythmia of guinea pigs were induced by isoprenaline.By using standard intracellular microelectrode technique to record Aps.Maximal diastolic potential(MDP),amplitude of action potential(APA),maximal rate of depolarization(Vmax),velocity of diastolic depolarization(VDD),50% and 90% of duration of action potential(APD 50,APD 90),rate of pacemaker firing(RPF).Results Perfusion ...