壳聚糖对人子宫内膜癌细胞影响的实验研究

目的:研究壳聚糖对人子宫内膜癌Ishikawa细胞的影响,为将壳聚糖做为宫内节育器材料提供依据。方法:以添加不同浓度壳聚糖培养的细胞为实验组,正常培养的细胞为空白对照组,使用添加20%胎牛血清和添加阿霉素培养的细胞为阳性对照组。各组细胞处理48h后,噻唑蓝法测定细胞增殖活性及毒性,5-溴脱氧尿嘧啶核苷掺入法测定DNA合成情况,流式细胞仪检测细胞周期变化。结果:与空白对照组比较,在壳聚糖浓度≤10μg/ml时各实验组细胞增殖能力无明显变化(P﹥0.05),细胞DNA合成速率无明显影响(P﹥0.05);壳聚糖浓度≤100μg/ml无明显的细胞毒性产生;在10μg/ml浓度壳聚糖组细胞周期分析时G1期、G2期、S期均无明显变化(P﹥0.05)。结论:壳聚糖浓度在<10μg/ml对Ishikawa细胞的增殖生长、DNA合成与细胞周期均无明显影响,≤100μg/ml未见细胞毒性产生。壳聚糖具有作为宫内节育器的生物缓释材料使用的前景。

Cytotoxicity of Chitosan on human endometrial adenocarcinoma cell

Objective:To study the effects of Chitosan on endometrial carcinoma cell lines in vitro.Methods:Chitosan at different concentrations were added to the culture medium of endometrial cancer cell line Ishikawa,as the experiment groups,while 20% FBS and 5μg/ml adriamycin added as positive control,and there was another blank control group with nothing added.Each group was treated for 48 hours.Cell proliferation was determined by MTT assays and BrdU incorporation ELISA.The apoptosis rate and cell cycle in Ishikawa cells were detected by flow cytometry.Results:When treated with 10μg/ml chitosan for 48h,the growth of Ishikawa cells was not significantly different from that of blank control group(P>0.05).Treatment with ≤10μg/ml chitosan for 48 hours did not significantly alter Ishikawa cell DNA synthesis when compared with the control(P>0.05).Chitosan at the concentration of ≤100μg/ml showed no significant cytotoxicity.Flow cytometry showed that 10μg/ml chitosan had no significant impact on the S and G1 and G2 Phases of cell cycle compared with blank control group(P>0.05).Conclusion:Chitosan at concentration of less than 10μg/ml had no effects on Ishikawa cell proliferation and cycling.It is indicated that chitosan can be used as drug carrier material in intrauterine device(IUD).