用不同实验小鼠品系对10个基因进行功能复筛的研究

作者利用反义核酸技术建立了用于神经系统新基因功能筛选研究用的小鼠行为学检测平台，用BALB／c小鼠对38个小鼠的新基因功能进行了初步筛选，又从中选取了10个与生理盐水（saline)及随机序列核酸（scramble)两个对照组都具有统计学差异的基因，进一步使用C57BL／6和ICR小鼠进行了复筛。实验结果发现，各品系小鼠的表现不尽相同。初筛结果显示：10个基因中与记忆相关的有6个，与痛觉相关的有2个，另2个与运动相关。但复筛的实验结果为：与记忆相关的只有3个（Am,L和Sn)，与痛觉相关的有2个（Pe和Rp)。研究说明了用不同品系进行复筛的重要性，也说明了在基因功能筛选实验中，使用近交系实验小鼠优于远交群。研究不同品系在同一实验中的不同表现，同研究用不同品系来验证同一实验结果一样，对于动物实验和实验动物科学的发展都有着重大的理论意义和现实意义。

Study on the Rescreening of 10 Genes' Functions Using Different Laboratory Mouse Strains

Employing the technology of antisense oligo nucleotide acid, the authors established a mouse behavior monitoring platform aimed at the large scale screening of novel gene functions in CNS. And with this platform, 38 genes have been screened using BALB/c mice. Among them, 10 genes showed differences to the control groups (saline, scramble). The authors screened the 10 genes again with C57BL/6 and ICR mice, and found the appearances were not always the same in three strains. In the first round screening, there are 6 of the 10 genes related to memory, 2 related to pain sensitivity, and the other 2 related to locomotion activity. But after the rescreening, only 3 genes show relativity with memory (Am, L, and Sn), and 2 genes show relativity with pain sensitivity (Pe, Rp). The results indicate that the importance of using different strains in the experiments, and it is better to use inbred strains than outbred colonies in the screening of gene functions. The results suggested that the research on different appearances of different mouse strains in one experiment and the use of different mouse strains to testify one result are both having very important significance to animal experiments and laboratory animal science.