金思维对脑缺血再灌模型大鼠学习记忆障碍的影响

目的:观测金思维对脑缺血大鼠空间学习记忆能力的影响。方法:金思维治疗脑缺血大鼠1个月后利用Morris水迷宫检测大鼠空间学习记忆能力并与都可喜对照。结果:1个月后,Morris水迷宫定位航行试验结果显示,从第3次游泳训练开始,金思维组、都可喜组和假手术组大鼠的逃避潜伏期均较模型组大大缩短,分别为(54.1±43.94),(55.9±43.49),(50.4±34.99),(85.4±42.8)s。第6次游泳训练后,金思维组(37.8±38.69)s、都可喜组(37.4±38.03)s、假手术组(26.9±21.63)s,大鼠的逃避潜伏期较模型组大鼠(77.5±47.59)s显著缩短,P<0.05;各组大鼠游泳路径比较结果同逃避潜伏期。空间探索试验中平台象限游泳时间和路径与总游泳时间和总路径之比结果显示,金思维组、都可喜组、假手术组显著高于模型组,P<0.05。结论:金思维能明显改善脑缺血模型大鼠空间学习记忆能力,为其改善脑缺血病人学习记忆能力的临床应用提供了药效学依据。

Research of Kingsbrain on improving the learning and memory ability of MCAO rats

Objective: To explore the influence of Kingsbrain(GETO) on the learning memory impairment of rats with cerebral ischemia.Method: Rats with cerebral ischemia were administered GETO orally once a day for one month.The ability of spatial-learning memory of rats was evaluated by Morris Water Maze(MWM).Duxil was used as a positive control.Result: the results of place navigation of MWM showed that at the 3~(rd) time of swimming training,the escape latency of rats of the GETO group,Duxil group and Sham group were shorter than that of model group.The escape latency were(54.1±43.94),(55.9±43.49),(50.4±34.99)and(85.4±42.8) s,respectively;but there was no significantly difference.After the 6~(th) time of swimming training,the escape latency of rats of the GETO group(37.8±38.69) s,the Duxil group(37.4±38.03) s and the sham group(26.9±21.63) s were significantly shorter than that of model rats(77.5±47.59) s,(P<0.05,) respectively.Comparison of the swimming distance among groups were similar to the escape latency among groups.In the test of spatial probe,results of the ratio of the swimming time of platform quadrant(tP) vs the total swimming time(tT) and the ratio of the swimming distance of platform quadrant(dP) vs the total swimming distance(dT) indicated that the ratios of the GETO group(0.347±0.066 2,0.344±0.055 1),the Duxil group(0.345±0.098 4,0.34±0.093 4) and the sham group(0.35±0.066 2,0.349±0.058 9) were significantly higher than those of the Model group(0.261±0.068 9,0.274±0.054 4),P<0.05,respectively.Conclusion: GETO can significantly improve the spatial learning and memory ability of rats with cerebral ischemia,which provides the pharmacodynamics evidence for its clinical application of improveing the learning and memory ability in poststroke patients.