不同剂量地塞米松对兔海水淹溺性肺水肿细胞因子变化的影响

目的：探讨地塞米松对兔海水淹溺性肺水肿（PE-SWD）模型的治疗作用及其对血清IL-8、TNF-α及IL-10的影响。方法：40只家兔随机分为对照组（CG,10只）和不同剂量（0.05 mg/kg、0.2 mg/kg、1.0 mg/kg）地塞米松治疗组（DG1、DG2、DG3,各10只）。用酶联免疫法（ELISA）测定淹溺前及淹溺后30、120 min血清中IL-8、TNF-α及IL-10的含量,并进行动脉血气分析。结果：与CG组比较,DG2、DG3组PaO2升高（P〈0.05）,IL-8、TNF-α含量显著下降（P〈0.01）,IL-10含量明显增加（P〈0.01）;DG2组与DG3组比较,PaO2、IL-8、TNF-α、IL-10无明显变化（P〉0.05）。结论：较大剂量地塞米松能减轻肺损伤,其机制可能与抑制IL-8、TNF-α的释放,增加IL-10的生成有关。但增加剂量（1 mg/kg）并不能增加治疗效果。

Effects of dexamethasone on cytokine levels of rabbits with pulmonary edema induced by seawater drowning

Objective：To investigate the therapeutical effect of dexamethasone on rabbits with pulmonary edema induced by seawater drowning and detect the changes of serum IL-8,TNF-α and IL-10 levels.Methods： Forty rabbits were randomly divided into 4 groups： control group（CG）,dexamethasone group（0.05 mg/kg as DG1,0.2 mg/kg as DG2,1 mg/kg as DG3）.The levels of serum IL-8,TNF-α,and IL-10 were examined by ELISA.Arterial blood gas was analyzed.Results：Compared with CG,the PaO2 in DG2 and DG3 was increased（P0.05）,the serum IL-8 and TNF-α levels were decreased（P0.01）,the serum IL-10 levels was increased（P0.01）.There was no significant difference between DG2 and DG3（P0.05）.Conclusion：Dexamethasone at comparatively high dose attenuates lung injury after seawater drowning,which may be related to the IL-8,TNF-α reduction and IL-10 increase.However,higher dose（1 mg/kg） can not improve the treatment effect.