实验性大鼠脑出血血肿周围组织胶质细胞纤维酸性蛋白的动态表达

目的:动态观察胶质细胞纤维酸性蛋白(GFAP)在脑出血动物模型脑组织中的表达及其意义。方法应用脑立体定向技术,建立大鼠脑出血模型,假手术作为对照组。分别测定脑组织含水量,观察血肿周围GFAP的表达规律。结果:大鼠脑出血后脑水肿于48h达到高峰,与对照组比较有统计学意义(P<0.05)。脑出血后脑内GFAP阳性细胞数6h表达最强,在72h时表达最少,7d的阳性细胞数量比72h增加(P<0.05),但数量少于6h(P<0.05)。相应时间点出血组阳性细胞数与对照组比较,有明显统计学意义,(P<0.05)。结论:脑出血后脑内GFAP的表达与脑水肿相关,提示星形胶质细胞在脑出血的病理变化过程中有重要作用。星形胶质细胞在损伤的修复作用具有二重性,即可以抑制也可以促进损伤修复。

The dynamic expression of Glial Fibrillary Acidic protein in peri-cerebral Hemorrhage of rat model

Objective: The purpose of this research is to investigate the role of glial fibrillary acidic protein (GFAP) in intracerebral hemorrhage (ICH) of animals model.Methods:ICH rat model was made by stereotactic technology, and sham injections served as controls. We measured brain water content, and GFAP expression after ICH.Results: The measured results show that the brain edema achieves the peak value 48 hours after ICH. The brain edema is statistically significantly higher than that of the comparison group (P<0.05). The number of the masculine cells with obvious GFAP immunity reaction around haematoma area was observed to achieve the highest at 6 hours and the lowest at 72 hour after ICH. Seven days after ICH, the quantity of masculine cells increased significantly comparing to that after 72 hours (P<0.05) but less than the quantity after 6 hours (P<0.05). The ICH group of those masculine cells with the comparison group at different times demonstrates the validation of this study method statistically (P<0.05).Conclusion: The GFAP reactions in intracerebral hemorrhage area are highly related to brain edema, indicating that astrocyte plays a pivotal role in the pathological change of intracerebral hemorrhage. It either restrains or expedites the reparation of the intracerebral hemorrhage.