| 六味地黄汤免疫调节活性成分化学分离的药理学导向评价 |
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| 引用本文： | 聂伟,张永祥,茹祥斌,乔善义,姚新生,周金黄. 六味地黄汤免疫调节活性成分化学分离的药理学导向评价[J]. 中国中西医结合杂志, 1998, 18(5): 287-289 |
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| 作者姓名： | 聂伟 张永祥 茹祥斌 乔善义 姚新生 周金黄 |
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| 作者单位： | 军事医学科学院毒物药物研究所!北京100850(聂伟，张永祥，茹祥斌，周金黄)，沈阳药科大学(乔善义，姚新生) |
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| 基金项目： | 国家自然科学基金资助项目!(No 395 70 85 1) |
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| 摘 要： | 为定向追踪分离六味地黄汤发挥免疫调节作用的活性成分进行导向评价。方法；运用化学与药理学密切合作的研究方法，以环磷酰胺处理小鼠为模型，抗体生成反应为活性评价指标，对每一步化学分离所得组分进行活性评价，确定活性部位。
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| 关 键 词： | 六味地黄汤 免疫调节 活性成分 化学分离 药理学 |
Guiding Evaluation of Immunomodulating Activity of the Liuwei Dihuang Decoction During the Stepwise Fractionation |
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| Nie Wei,Zhang Yongxiang,Ru Xiangbin,et al Beijing Institute of Pharmacology and Toxicology,Beijing. Guiding Evaluation of Immunomodulating Activity of the Liuwei Dihuang Decoction During the Stepwise Fractionation[J]. Chinese journal of integrated traditional and Western medicine, 1998, 18(5): 287-289 |
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| Authors: | Nie Wei Zhang Yongxiang Ru Xiangbin et al Beijing Institute of Pharmacology Toxicology Beijing |
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| Abstract: | Objective: To conduct the activity evaluation for immunomodulating components from the Liuwei Dihuang Decoction (LWDHD). Methods: Cyclophosphamide (Cy) treated mice were used as the immunodeficient model and the antibody production response in plaque forming cell (PFC) assay was employed as the activity evaluating parameter. Stepwise fractionation was guided by activity evaluation. Results: Fr1, the ethanol soluble fraction, and Fr2, the ethanol insoluble fraction of LWDHD, both significantly improved the antibody production response in Cy treated mice, in which Fr2 showed stronger activity than that of Fr1. Fr2 was further fractionated. Fr2 CA4, one of the fractions obtained by active carbon chromatography from Fr2, exhibited the strongest activity in comparison with other fractions. Conclusion: CA4, which is mainly composed of acidic polysaccharides, is the main immunomodulating active fraction contained in the ethanol insoluble fraction of LWDHD. |
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| Keywords: | Liuwei Dihuang Decoction immunomodulating effect active fraction chemical fractionation |
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