雌激素受体和孕激素受体在女性牙周炎患者牙龈组织中的表达

目的观察比较女性牙周炎患者及正常女性牙龈组织雌激素(ER)和孕激素(PR)的表达分布,为进一步阐明ER和PR与女性牙周炎的关系提供实验依据。方法收集正常对照组牙龈标本30例,中、重度牙周炎组34例,按年龄各自分成3组,标本行免疫组化处理(Envision法),显微镜下观察,统计分析。结果ER和PR在细胞胞浆及胞核中均有表达,无规律地分布于牙龈上皮层中,阳性样本的染色强度强弱不一。正常女性牙龈组织中ER及PR阳性率较低,女性牙周炎患者牙龈组织中ER阳性率明显升高(P<0.01),PR阳性率无明显改变(P>0.05),与年龄无明显关系。结论女性牙龈组织中ER的表达可能与牙周炎相关,雌激素对牙龈组织除了起间接作用外,还可通过ER直接影响牙周组织;而孕激素对牙周组织可能以间接影响为主。

Expression of Estrogen and Progesterone Receptors in the Gingival Tissues of Female Patients with Moderate and Advanced Periodontitis

Objective The purpose of this study was to compare the difference of sexual hormone receptors' expression and distribution in the gingival tissues between periodontitis patients and normal females and to provide the theoretical facts for the clarification of periodontitis's pathogenesis.Methods The test group was34samples and normal control group was30samples.The test group and the normal control group were divided into3subgroups separately according to age.All specimens were studied for ER and PR proteins' expression using immunohistochemical techniques(Envision)uniformly,and observed by light microscope.Results ER and PR proteins located in cytoplasm or nuclear.ER and PR proteins' stainings cccured in the epithelium,the distribution of them had no regularity and staining intensity varied.The positive rates of ER and PR in the gingival tissues of normal female were low.The positive rate of ER in the gingival tissues of female with periodontitis soared apparently(P<0.01),but the positive rate of PR changed little(P>0.05).Conclusion The expression of ER in the gingival tissues might have relation to periodontitis of females.Besides acting indirectly on the gingival tissues,estrogen could affect the gingival tissues directly by ER;progesterone might have indirect effects on gingival tissues.