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Determination of serum cholesterol concentration in the presence of ascorbate.
Authors:P J Lumb and B M Slavin
Affiliation:Department of Endocrinology & Chemical Pathology, St Thomas''s Hospital, London.
Abstract:Pretreatment of a serum or plasma sample with ascorbate oxidase removed interfering ascorbate and allowed the determination of cholesterol to be carried out by a current enzymatic cholesterol method available in kit form. The Cobas-Fara was programmed to carry out pretreatment of the sample with ascorbate oxidase before addition of the cholesterol colour reagent.
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