Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport. |
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Authors: | P J Krysan J C Young F Tax and M R Sussman |
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Institution: | Cell and Molecular Biology Program and the Department of Horticulture, University of Wisconsin, Madison 53706, USA. |
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Abstract: | The transferred DNA (T-DNA) of Agrobacterium tumefaciens serves as an insertional mutagen once integrated into a host plant's genome. As a means of facilitating reverse genetic analysis in Arabidopsis thaliana, we have developed a method that allows one to search for plants carrying F-DNA insertions within any sequenced Arabidopsis gene. Using PCR, we screened a collection of 9100 independent T-DNA-transformed Arabidopsis lines and found 17 T-DNA insertions within the 63 genes analyzed. The genes surveyed include members of various gene families involved in signal transduction and ion transport. As an example, data are shown for a T-DNA insertion that was found within CPK-9, a member of the gene family encoding calmodulin-domain protein kinases. |
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