| 磁性纳米四氧化三铁及纳米金协同柔红霉素作用于K562/A02细胞的初步研究 |
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| 引用本文： | 戴永援,陈宝安,王雪梅,张仁云,高峰,丁家华,高冲,孙耘玉,程坚,赵刚,王骏.磁性纳米四氧化三铁及纳米金协同柔红霉素作用于K562/A02细胞的初步研究[J].东南大学学报(医学版),2007,26(3):157-160. |
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| 作者姓名： | 戴永援 陈宝安 王雪梅 张仁云 高峰 丁家华 高冲 孙耘玉 程坚 赵刚 王骏 |
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| 作者单位： | 东南大学附属中大医院,血液科,江苏,南京,210009;东南大学,生物电子学国家重点实验室,江苏,南京,210096 |
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| 基金项目： | 国家自然科学基金
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东南大学校科研和教改项目 |
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| 摘 要： | 目的：研究磁性纳米四氧化三铁（Nano-Fe3O4）及纳米金（Nano-Au）是否具有逆转K562/A02细胞耐药的作用。方法：用MTT法测定盐酸柔红霉素（DNR）对K562/A02和K562细胞的毒性,并观察DNR与Nano-Fe3O4或Nano-Au的联合作用。结果：体积分数为25%的Nano-Fe3O4及Nano-Au能有效增强DNR的细胞毒作用,提高细胞的凋亡率,差异具有显著性。结论：Nano-Fe3O4及Nano-Au结合DNR后均能有效逆转K562/A02细胞的耐药性。
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| 关 键 词： | 磁性纳米四氧化三铁 纳米金 柔红霉素 多药耐药 K562/A02细胞 |
| 文章编号： | 1671-6264(2007)03-0157-04 |
| 修稿时间： | 2006年9月28日 |
Synergistic effect of magnetic nanopartical Fe3O4,Au and daunomycin on K562/A02 |
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| DAI Yong-yuan,CHEN Bao-an,WANG Xue-mei,ZHANG Ren-yun,GAO Feng,DING Jia-hua,GAO Chong,SUN Yun-yu,CHENG Jian,ZHAO Gang,WANG Jun.Synergistic effect of magnetic nanopartical Fe3O4,Au and daunomycin on K562/A02[J].Journal of Southeast Univ: Medical Sci Ed,2007,26(3):157-160. |
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| Authors: | DAI Yong-yuan CHEN Bao-an WANG Xue-mei ZHANG Ren-yun GAO Feng DING Jia-hua GAO Chong SUN Yun-yu CHENG Jian ZHAO Gang WANG Jun |
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| Abstract: | Objective To determine whether the magnetic Nano-Fe3O4 and Nano-Au can reverse the resistance of K562/A02 cells or not.Methods MTT was used to examine the effects of daunomycin with and without the magnetic Nano-Fe3O4 or Nano-Au on K562 and K562/A02 cells.The intracellular free daunomycin concentration and the apoptosis percentage of the K562/A02 cells were measured by flow cytometry and fluorescent microscope.Results Nano-Fe3O4 or Nano-Au at a 25% volumn ratio raised the intracellular daunomycin accumulation of the K562/A02 cells and induced more apoptosis percentage.Conclusion The Nano-Fe3O4 or Nano-Au with daunomycin can reverse the resistance of K562/A02 cells significantly. |
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| Keywords: | Nano-Fe3O4 Nano-Au daunomycin multidrug resistance K562/A02 cells |
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