雄激素干预对PC12细胞Seladin-1表达的影响 |
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引用本文: | 邬军锋,祖衡兵,陈英辉,张建峰,王翠翠,王子高. 雄激素干预对PC12细胞Seladin-1表达的影响[J]. 神经疾病与精神卫生, 2010, 10(2): 119-121. DOI: 10.3969/j.issn.1009-6574.2010.02.003 |
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作者姓名: | 邬军锋 祖衡兵 陈英辉 张建峰 王翠翠 王子高 |
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作者单位: | 复旦大学附属金山医院神经内科,200540 |
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摘 要: | 目的观察雄激素干预对体外培养PC12细胞Seladin-1水平的影响。方法将培养的PC12细胞分为5组:正常对照组、药物处理组(雄激素终浓度分别为1×10-3mol/L、1×10-4mol、1×10-5mol/L、1×10-6mol/L)。采用Western-blot和Real-time PCR方法对PC12细胞Seladin-1的表达进行检测。结果各药物组处理后,PC12细胞Seladin-1的蛋白质与mRNA的表达水平与正常对照组比较均明显提高,差异具有统计学意义(P〈0.05,P〈0.001)。结论雄激素对PC12细胞Seladin-1基因的表达具有正性调控作用。雄激素的神经保护效应可能与上调seladin-1的表达有关。
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关 键 词: | PC12细胞 雄激素类 Seladin-1 基因表达 |
Effects of androgen on the expression of Seladin-1 in PC12 cells |
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Affiliation: | WU Jun-feng, ZU Heng-bing, CHEN Ying-hui, et al. Department of Neurology, the Affiliated Jinshan Hospital of Fudan Uni- versity, Shanghai 200540, China |
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Abstract: | Objective To investigate the effects of androgen on the expression of Seladin--1 in PC12 cells. Methods The effects of different concentrations of androgen on the expression of Seladin--1 in PC12 cells were determined by Western blot analysis and Real--time PCR. Results The expression of Seladin--1 was significantly increased in the levels of both protein and mRNA (P 〈 0.05, P 〈 0. 001). Conclusions Androgen can positively modulate the expression of Seladin--1. The neuroprotective effect of androgen might be mediated by the up-- regulation of Seladin-- 1 expression. |
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Keywords: | Seladin-1 |
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