Factors affecting 39K NMR detectability in rat tissue |
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Authors: | S A Rashid W R Adam D J Craik B P Shehan R M Wellard |
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Affiliation: | School of Pharmaceutical Chemistry, Victorian College of Pharmacy Ltd, Parkville, Australia. |
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Abstract: | In this study we have found that NMR detectability of 39K in rat thigh muscle may be substantially higher (up to 100% of total tissue potassium) than values previously reported of around 40%. The signal was found to consist of two superimposed components, one broad and one narrow, of approximately equal area. Investigations involving improvements in spectral parameters such as signal-to-noise ratio and baseline roll, together with computer simulations of spectra, show that the quality of the spectra has a major effect on the amount of signal detected, which is largely due to the loss of detectability of the broad signal component. In particular, lower-field spectrometers using conventional probes and detection methods generally have poorer signal-to-noise and worse baseline roll artifacts, which make detection of a broad component of the muscle signal difficult. |
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