METHODS FOR ESTIMATION OF BLOOD GLUCOSE : A COMPARATIVE EVALUATION |
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| Authors: | VIVEK N AMBADE YV SHARMA BL SOMANI |
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| Affiliation: | *Scientist C, Armed Forces Medical College, Pune 411040;+Professor and Head, Armed Forces Medical College, Pune 411040;#Scientist E, Dept of Biochemistry, Armed Forces Medical College, Pune 411040 |
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| Abstract: | Glucose in blood is the most frequent analyzed parameter in a clinical chemistry laboratory. In Armed Forces Laboratories, copper reduction method (Modified Folin Wu) is commonly used. Here we have compared this method as well as O-Toluidine and GOD-POD method with reference UV-Hexokinase method. Both Modified Folin Wu and O-Toluidine showed upward deviation with substantial imprecision (CV=4.9% – 3.5% and 6% – 5.8% respectively) and inaccuracy (Average deviation = 5.76% and 10.68% respectively). GOD-POD method was found linear (up to 500 mg/dl), with good precision (CV=0.7% to 1.4%) and accuracy (Average deviation= −0.97%). This method is simple, rapid, economical and sensitive, and can be adopted to a routine colorimeter.KEYWORDS: Blood glucose, Diabetes mellitus, Glucose oxidase |
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